Rabbit Anti-POU2AF1 Recombinant Antibody (clone 4A5) (CAT#: ZG-0516U)
Rabbit Anti-POU2AF1 Antibody (clone 4A5) displayed high specificity against the POU2AF1 protein of Human.
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WB
Figure 1 Rabbit Anti-POU2AF1 Antibody (ZG-0516U) in WB
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: BOB1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa
IHC
Figure 2 Rabbit Anti-POU2AF1 Antibody (ZG-0516U) in IHC
IHC image of ZG-0516U diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human BOB1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POU2AF1
- Species Reactivity
- Human
- Clone
- 4A5
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hodgkin's Lymphoma, Lymphocytic Depletion and Primary Biliary Cholangitis.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU2AF1; OBF1
- Gene ID
- 5450
- UniProt ID
- Q16633
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1507MZ | Recombinant Mouse Anti-Human POU2AF1 Antibody (clone Xvf-BD6) | IHC-P | Mouse antibody |
| ZG-0054J | Mouse Anti-POU2AF1 Recombinant Antibody (ZG-0054J) | IHC-P, IF | Mouse IgG |
| VS3-WK1013 | Mouse Anti-POU2AF1 Recombinant Antibody (VS3-WK1013) | IHC | Mouse IgG2a |
| VS3-WK1014 | Mouse Anti-POU2AF1 Recombinant Antibody (clone ABT-BOB1) | IHC-P | Mouse IgG2a, κ |
| VS3-XY3473 | Rabbit Anti-POU2AF1 Recombinant Antibody (clone R01-6M2) | WB, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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