CytoStream™ Rabbit Anti-POU2AF1 Recombinant Antibody (VS13-YC919) (CAT#: VS13-YC919)
The CytoStream™ Rabbit Anti-POU2AF1 Recombinant Antibody is specific for human POU2AF1. POU2AF1 enables transcription coactivator activity, regulating RNA polymerase II transcription as part of its regulator complex. The anti-POU2AF1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining shows high expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg
(Immunohistochemical staining shows high expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27284_B_8_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27284_B_8_5.jpg
Figure 3 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_4.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_5.jpg
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_4_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_8.jpg
Figure 8 Tonsil
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8.jpg
Figure 9 RNA cell line category: Group enriched (Daudi, Karpas-707, U-266/70, U-266/84, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110777-POU2AF1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110777-POU2AF1/subcellular
Specifications
- Immunogen
- Recombinant human BOB1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POU2AF1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- OCA-B, OBF1, BOB1, OCT-Binding Factor 1, POU Domain Class 2-Associating Factor 1
- Gene ID
- 5450
- UniProt ID
- Q16633
- Long Name
- POU Class 2 Homeobox Associating Factor 1
- Sequence Similarities
- Belongs to the POU2AF family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_006226.2
- Function
- Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290).
It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176).
It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176).
It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290).
Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2794 | Hi-Affi™ Recombinant Rabbit Anti-POU2AF1 Monoclonal Antibody (DS2794AB) | ICC, IHC-P, WB | IgG |
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