CytoStream™ Rabbit Anti-POU2AF1 Recombinant Antibody (VS13-YC919)
CAT#: VS13-YC919
The CytoStream™ Rabbit Anti-POU2AF1 Recombinant Antibody is specific for human POU2AF1. POU2AF1 enables transcription coactivator activity, regulating RNA polymerase II transcription as part of its regulator complex. The anti-POU2AF1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human tonsil
Immunohistochemical staining shows high expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27284_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (Daudi, Karpas-707, U-266/70, U-266/84, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110777-POU2AF1/subcellular
❮
❯
Specifications
- Immunogen
- Recombinant human BOB1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human POU2AF1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- OCA-B, OBF1, BOB1, OCT-Binding Factor 1, POU Domain Class 2-Associating Factor 1
- Gene ID
- 5450
- UniProt ID
- Q16633
- Long Name
- POU Class 2 Homeobox Associating Factor 1
- Sequence Similarities
- Belongs to the POU2AF family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_006226.2
- Function
- Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290).
It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176).
It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176).
It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290).
Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1507MZ | Recombinant Mouse Anti-Human POU2AF1 Antibody (clone Xvf-BD6) | IHC-P | Mouse antibody |
| ZG-0054J | Mouse Anti-POU2AF1 Recombinant Antibody (ZG-0054J) | IHC-P, IF | Mouse IgG |
| ZG-0516U | Rabbit Anti-POU2AF1 Recombinant Antibody (clone 4A5) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX894 | Mouse Anti-POU2AF1 Recombinant Antibody (clone 1170CT1.1.1) | WB | Mouse IgG1, κ |
| VS3-WK1013 | Mouse Anti-POU2AF1 Recombinant Antibody (VS3-WK1013) | IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2794 | Hi-Affi™ Recombinant Rabbit Anti-POU2AF1 Monoclonal Antibody (DS2794AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1934 | PE-B*51:01/Human POU2AF1 (DAYALNHTL) MHC Tetramer | FCM | |
| MHC-LC4449 | PE-B*15:01/Human POU2AF1 (LPHQPLATY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5579 | Anti-POU2AF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5580 | Anti-Mouse POU2AF1 Immunohistochemistry Kit | IHC |
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