R-Phycoerythrin AffiniPure F(ab')₂ Fragment Goat Anti-Human IgG, F(ab')₂ fragment specific
CAT#: MOB-0239MC
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against the Fc portion of human IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Specifications
- Host Species
- Goat
- Species Reactivity
- Human
- Conjugate
- R-Phycoerythrin
Product Property
- Purification
- The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
- Format
- Freeze-dried powder
- Buffer
- 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
- Storage
- Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day.
Applications
- Application Notes
- Flow Cytometry:- 1:50-1:200
Target
- Alternative Names
- F(ab')₂; fragment antigen-binding (Fab) fragment
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Bright and Reliable
This antibody has been a game-changer for our flow cytometry experiments. The R-PE conjugate is incredibly bright, allowing us to easily detect even low levels of our target. The F(ab')₂ specificity minimizes background noise, leading to clearer, more reliable data.
Easy to Use
This antibody is straightforward to work with. Its stability at 2-8°C and the clear guidelines provided made it easy to integrate into our protocols. The results have been consistently reliable across multiple experiments.
Q&As
-
What is the main use of this R-Phycoerythrin-AffiniPure F(ab')₂ Goat Anti-Human IgG antibody?
A: This antibody is primarily used for detecting human IgG F(ab')₂ fragments in various immunological assays. It's conjugated with R-Phycoerythrin (R-PE), a bright fluorescent dye, making it ideal for applications like flow cytometry and immunofluorescence where fluorescence detection is required.
-
Is this antibody cross-reactive with other species?
A: This antibody is goat-derived and specifically reacts with human IgG F(ab')₂ fragments. It does not significantly cross-react with IgG from other species, making it ideal for experiments requiring high specificity to human IgG.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Fab"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0361MC | Goat Anti-Human IgG Fab Antibody (HRP) | ELISA, WB, IHC, IF, ICC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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