What is the main use of this R-Phycoerythrin-AffiniPure F(ab')₂ Goat Anti-Human IgG antibody?

This antibody is primarily used for detecting human IgG F(ab')₂ fragments in various immunological assays. It's conjugated with R-Phycoerythrin (R-PE), a bright fluorescent dye, making it ideal for applications like flow cytometry and immunofluorescence where fluorescence detection is required.

Is this antibody cross-reactive with other species?

This antibody is goat-derived and specifically reacts with human IgG F(ab')₂ fragments. It does not significantly cross-react with IgG from other species, making it ideal for experiments requiring high specificity to human IgG.

R-Phycoerythrin AffiniPure F(ab')₂ Fragment Goat Anti-Human IgG, F(ab')₂ fragment specific

Product Property

Purification
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Format
Freeze-dried powder

Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Storage
Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Mouse Antibody Products

CAT	Product Name	Application	Type
MOB-0361MC	Goat Anti-Human IgG Fab Antibody (HRP)	ELISA, WB, IHC, IF/ICC

Customer Reviews and Q&As

Customer Review Q&As

Bright and Reliable

This antibody has been a game-changer for our flow cytometry experiments. The R-PE conjugate is incredibly bright, allowing us to easily detect even low levels of our target. The F(ab')₂ specificity minimizes background noise, leading to clearer, more reliable data.

Easy to Use

This antibody is straightforward to work with. Its stability at 2-8°C and the clear guidelines provided made it easy to integrate into our protocols. The results have been consistently reliable across multiple experiments.