Recombinant Human Anti-Human RPRD1A Antibody Fab Fragment, clone D223
CAT#: DrMAB-497
Recombinant Human Antibody binds selectively to Human RPRD1A, expressed in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/415_H9_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/411_H9_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/84769_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:High
Intensity: Strong
Enterocytes
Staining:High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:Medium
Intensity: Moderate
Goblet cells
Staining:High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/84769_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/84769_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/84769_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40602/84769_A_9_8.jpg
RNA Expression
Figure 10 NA cell line categoryi: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141425-RPRD1A
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Type
- Fab
- Specificity
- reacts with human RPRD1A
- Species Reactivity
- Human
- Clone
- D223
- Applications
- IP; IF; ELISA; IP-MS
Product Property
- Storage
- short term – store at 4°C (over 6 months), long term - PBS -20°C or -80°C
Target
- Alternative Names
- RPRD1A; regulation of nuclear pre-mRNA domain containing 1A; P15RS; HsT3101; regulation of nuclear pre-mRNA domain-containing protein 1A; CDKN2B-related protein; Cyclin-dependent kinase inhibitor 2B-related protein (p15INK4B-related protein); cyclin-depen
- Gene ID
- 55197
- UniProt ID
- A0A024RC37
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RPRD1A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-502 | Recombinant Human Anti-Human RPRD1A Antibody, clone D490 | ELISA, IP-MS | IgG |
| MOB-1426CT | Recombinant Mouse anti-Human RPRD1A Monoclonal antibody (EML330) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6204 | Anti-RPRD1A Immunohistochemistry Kit | IHC |
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