Recombinant Human Anti-human PRMT1 Antibody (CAT#: MHH-802)
Recombinant Human Antibody prepared by a genetic engineering technique which specifically reacts with Human PRMT1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-PRMT1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-PRMT1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72136/1476_A6_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72136/1476_A6_1_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69769/163033_B_7_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69769/163033_B_7_5_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_B_7_5.jpg
(Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_B_7_5.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_8_3.jpg
(Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_7_5.jpg
Figure 6 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_4_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126457-PRMT1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126457-PRMT1/subcellular
Specifications
- Immunogen
- Human protein arginine methyltransferase 1
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human protein arginine methyltransferase 1
- Species Reactivity
- Human
- Applications
- Antibody assay: Enzyme-linked Immunosorbent Assay; Immunoprecipitation; Functional Study
Product Property
- Purity
- 95% by HPLC
- Storage
- ≤6 months at 4°C; ≥6 months at -20°C.
Target
- Alternative Names
- PRMT1; protein arginine methyltransferase 1; ANM1; HCP1; IR1B4; HRMT1L2; protein arginine N-methyltransferase 1; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; heter
- Gene ID
- 3276
- UniProt ID
- A0A087X1W2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-802 | Recombinant Anti-human PRMT1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MOB-1690CT | Recombinant Mouse anti-Human PRMT1 Monoclonal antibody (NBU-C23) | ChIP, WB | |
| ZG-0399C | Mouse Anti-PRMT1 Recombinant Antibody (ZG-0399C) | WB | Mouse IgG |
| ZG-0400C | Mouse Anti-PRMT1 Recombinant Antibody (ZG-0400C) | WB | Mouse IgG |
| VS3-FY1200 | Recombinant Rabbit Anti-PRMT1 Antibody (clone R08-3H8) | WB, IHC-P | Rabbit IgG |
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