Mouse Anti-NHP PRMT1 Recombinant Antibody (clone PRMT1-171) (CAT#: VS-1024-XY408)
This exemplary monoclonal antibody is engineered to target PRMT1, exhibiting broad reactivity across a diverse range of species, including bovine, dog, chicken, hamster, human, mouse, non-human primate, and rat. Utilizing full-length PRMT1 as its immunogen ensures optimal specificity and binding affinity. Suitable for applications in Western blotting and immunocytochemistry, this antibody serves as a crucial tool for exploring the multifaceted roles of PRMT1 in cellular processes and disease mechanisms. Its versatility and precision make it an indispensable resource for researchers dedicated to advancing our understanding of protein arginine methylation.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72136/1476_A6_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72136/1476_A6_1_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69769/163033_B_7_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69769/163033_B_7_5_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_B_7_5.jpg
(Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_B_7_5.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_8_3.jpg
(Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_7_5.jpg
Figure 6 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_4_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126457-PRMT1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126457-PRMT1/subcellular
Specifications
- Immunogen
- Full length PRMT1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PRMT1
- Species Reactivity
- Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat
- Clone
- PRMT1-171
- Applications
- WB, ICC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 2 µg/mL, ICC: 5 µg/mL
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-802 | Recombinant Anti-human PRMT1 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-802 | Recombinant Human Anti-human PRMT1 Antibody | ELISA, IP, FuncS | IgG |
| MOB-1690CT | Recombinant Mouse anti-Human PRMT1 Monoclonal antibody (NBU-C23) | ChIP, WB | |
| MRO-1267-CN | Recombinant Rabbit Anti-PRMT1 Monoclonal Antibody (JA11-17) | WB, IF, IHC, ChIP | Rabbit IgG |
| VS3-FY1200 | Recombinant Rabbit Anti-PRMT1 Antibody (clone R08-3H8) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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