Z-domain of protein A anti-gp120 (SZA-L221)
This gp120 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=100 nM for the univalent scaffold proteins and 10 nM for the bivalent scaffold proteins) for gp120 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specifications
- Scaffold Name
- Z-domain of protein A
- Origin
- Staphylococcal protein A
- Core Structure
- 3-α helixes
- Variable Regions
- 13 Residues on first and second helix surface
- Target
- gp120
- Species Reactivity
- HIV-1
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 100 nM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
- Alternative Names
- Staphylococcal protein A; Z-domain of protein A; HIV1gp8
- Entrez Gene ID
- 155971
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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