Z-domain of protein A scaffold protein anti-HIV-1 gp120 (CAT#: SZA-L221)

This gp120 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=100 nM for the univalent scaffold proteins and 10 nM for the bivalent scaffold proteins) for gp120 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.