APC-A*29:02/Human Ubiquitin (KESTLHLVL) MHC Tetramer (CAT#: MHC-CN1298)

1. Q 1: What are common applications for this MHC tetramer?

   A: Applications include identifying and quantifying antigen-specific T cells, monitoring immune responses, and studying T-cell receptor specificity using flow cytometry.

2. Q 2: What is the expected staining pattern with this tetramer?

   A: Expect specific staining of T cells that have T-cell receptors (TCRs) recognizing the KESTLHLVL peptide presented by HLA-A*29:02. Non-specific staining should be minimal with proper controls.

3. Q 3: Are there recommended blocking steps to reduce non-specific binding?

   A: A blocking step using human serum or an Fc receptor blocking agent can minimize non-specific binding. This step is typically done before adding the tetramer to the cell suspension.

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