Recombinant Human Anti-AAV Antibody (D3) (CAT#: HPAB-1540LY)

This product is antibody D3 recognizes AAV. The antibody D3 immunoassay techniques such as: ELISA.


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  • Datasheet
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Specifications

  • Host Species
  • Human
  • Specificity
  • AAV
  • Species Reactivity
  • AAV
  • Clone
  • D3
  • Applications
  • IF, ELISA

Product Property

  • Purification
  • 95% pure by SDS-PAGE
  • Purity
  • > 95% Purity determined by SDS-PAGE.
  • Format
  • Liquid
  • Buffer
  • PBS, pH 7.4
  • Storage
  • Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Applications

  • Application Notes
  • For Enzyme-linked Immunosorbent Assay: Binding of MAbs to AAV serotypes and AAV-2 insertion mutants was quantImmunofluorescenceied in an Enzyme-linked Immunosorbent Assay. Titers of determined CsCl-purImmunofluorescenceied particles of AAV serotypes and AAV-2 insertion mutants were determined by counting of negatively stained particles in electron micrographs. Particles were diluted in PBS, and equal amounts (8 × 10⁷ particles/well) were used to coat the wells of microtiter plates overnight at 4°C. After blocking, hybridoma supernatants diluted in PBS containing 0.05% Tween 20 were incubated for 1 h at 37°C, followed by another hour of incubation with a biotinylated anti-mouse secondary antibody. Detection and quantImmunofluorescenceication were performed as described previously.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone D3"

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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