Recombinant Human Anti-AAV Antibody (D3)
CAT#: HPAB-1540LY
This product is antibody D3 recognizes AAV. The antibody D3 immunoassay techniques such as: ELISA.
Specifications
- Host Species
- Human
- Specificity
- AAV
- Species Reactivity
- AAV
- Clone
- D3
- Applications
- IF, ELISA
Product Property
- Purification
- 95% pure by SDS-PAGE
- Purity
- > 95% Purity determined by SDS-PAGE.
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Applications
- Application Notes
- For Enzyme-linked Immunosorbent Assay: Binding of MAbs to AAV serotypes and AAV-2 insertion mutants was quantImmunofluorescenceied in an Enzyme-linked Immunosorbent Assay. Titers of determined CsCl-purImmunofluorescenceied particles of AAV serotypes and AAV-2 insertion mutants were determined by counting of negatively stained particles in electron micrographs. Particles were diluted in PBS, and equal amounts (8 × 10⁷ particles/well) were used to coat the wells of microtiter plates overnight at 4°C. After blocking, hybridoma supernatants diluted in PBS containing 0.05% Tween 20 were incubated for 1 h at 37°C, followed by another hour of incubation with a biotinylated anti-mouse secondary antibody. Detection and quantImmunofluorescenceication were performed as described previously.
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-1540LY, RRID: AB_3111420)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone D3"
- CAT
- Product Name
See other products for "AAVS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1940z-F(E) | Recombinant Anti-Human AAVS1 Antibody Fab Fragment | Neut, IF, FuncS | Fab |
| HPAB-1532LY-F(E) | Human Anti-AAV Antibody Fab Fragment (G3) | ELISA | |
| HPAB-1533LY-F(E) | Human Anti-AAV Antibody Fab Fragment (C8) | ELISA | |
| HPAB-1534LY-F(E) | Human Anti-AAV Antibody Fab Fragment (C11) | ELISA | |
| HPAB-1535LY-F(E) | Human Anti-AAV Antibody Fab Fragment (D10) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1532LY-S(P) | Human Anti-AAV Antibody scFv Fragment (G3) | ELISA | |
| HPAB-1533LY-S(P) | Human Anti-AAV Antibody scFv Fragment (C8) | ELISA | |
| HPAB-1534LY-S(P) | Human Anti-AAV Antibody scFv Fragment (C11) | ELISA | |
| HPAB-1535LY-S(P) | Human Anti-AAV Antibody scFv Fragment (D10) | ELISA | |
| HPAB-1536LY-S(P) | Human Anti-AAV Antibody scFv Fragment (F4) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1532LY | Recombinant Human Anti-AAV Antibody (G3) | ELISA | |
| HPAB-1533LY | Recombinant Human Anti-AAV Antibody (C8) | ELISA | |
| HPAB-1534LY | Recombinant Human Anti-AAV Antibody (C11) | ELISA | |
| HPAB-1535LY | Recombinant Human Anti-AAV Antibody (D10) | ELISA | |
| HPAB-1536LY | Recombinant Human Anti-AAV Antibody (F4) | ELISA |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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