Recombinant Human Anti-AAV Antibody (D3) (CAT#: HPAB-1540LY)
This product is antibody D3 recognizes AAV. The antibody D3 immunoassay techniques such as: ELISA.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Specifications
- Host Species
- Human
- Specificity
- AAV
- Species Reactivity
- AAV
- Clone
- D3
- Applications
- IF, ELISA
Product Property
- Purification
- 95% pure by SDS-PAGE
- Purity
- > 95% Purity determined by SDS-PAGE.
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Applications
- Application Notes
- For Enzyme-linked Immunosorbent Assay: Binding of MAbs to AAV serotypes and AAV-2 insertion mutants was quantImmunofluorescenceied in an Enzyme-linked Immunosorbent Assay. Titers of determined CsCl-purImmunofluorescenceied particles of AAV serotypes and AAV-2 insertion mutants were determined by counting of negatively stained particles in electron micrographs. Particles were diluted in PBS, and equal amounts (8 × 10⁷ particles/well) were used to coat the wells of microtiter plates overnight at 4°C. After blocking, hybridoma supernatants diluted in PBS containing 0.05% Tween 20 were incubated for 1 h at 37°C, followed by another hour of incubation with a biotinylated anti-mouse secondary antibody. Detection and quantImmunofluorescenceication were performed as described previously.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-1540LY, RRID: AB_3111420)
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For Research Use Only. Not For Clinical Use.
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