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Rabbit Anti-ACAT2 Recombinant Antibody (clone R88-4C-2) (CAT#: VS3-FY2155)

The anti-ACAT2 antibody is a rabbit recombinant monoclonal antibody that binds to human ACAT2. It cross-reacts with mouse, rat. Western blot demonstrated that R88-4C-2 recognized a target protein with a molecular weight of 41 kDa.


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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25736/342_E9_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_B_7_5.jpg
Figure 3 Colon
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25736/56966_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_9_4.jpg
Figure 5 Testis
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_4_6.jpg
Figure 6 Ovary
Figure 6 Ovary
(Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_4_7.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120437-ACAT2

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ACAT2
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R88-4C-2
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 38kDa.
  • Related Disease
  • Alpha-Methylacetoacetic Aciduria and Acetyl-Coa Acetyltransferase-2 Deficiency

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200

Target

  • Alternative Names
  • Acetyl-CoA Acetyltransferase 2; EC 2.3.1.9; Acetyl-Coenzyme A Acetyltransferase 2 (Acetoacetyl Coenzyme A Thiolase); Acetyl-Coenzyme A Acetyltransferase 2; Acetyl-CoA Transferase-Like Protein; Cytosolic Acetoacetyl-CoA Thiolase; Acetoacetyl Coenzyme A Thiolase; EC 2.3.1; ACTL
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • Ubiquitination at Lys124 and Lys235 (NX_Q9BWD1 [NX_Q9BWD1-1])
    Modification sites at PhosphoSitePlus (Q9BWD1)
    Glycosylation from GlyGen (Q9BWD1) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Involved in the biosynthetic pathway of cholesterol.

Recommended Products

Isotype Control

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ACAT2"

Recombinant Antibody

CAT Product Name Application Type
MOB-3714z Mouse Anti-ACAT2 Recombinant Antibody (clone 29H5) WB, IP, ELISA Mouse IgG2a, κ

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0012MZ Chicken Anti-Acetyl-CoA Acetyltransferase 2 Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0032 Hi-Affi™ Rabbit Anti-ACAT2 Recombinant Antibody (clone DS32AB) WB, IHC, IP, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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