Rabbit Anti-ACAT2 Recombinant Antibody (clone R88-4C-2)
CAT#: VS3-FY2155
The anti-ACAT2 antibody is a rabbit recombinant monoclonal antibody that binds to human ACAT2. It cross-reacts with mouse, rat. Western blot demonstrated that R88-4C-2 recognized a target protein with a molecular weight of 41 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25736/342_E9_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25736/56966_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_9_4.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_4_6.jpg
Normal Tissue
Figure 6 Ovary
Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_4_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25765/56974_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120437-ACAT2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACAT2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R88-4C-2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 38kDa.
- Related Disease
- Alpha-Methylacetoacetic Aciduria and Acetyl-Coa Acetyltransferase-2 Deficiency
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Acetyl-CoA Acetyltransferase 2; EC 2.3.1.9; Acetyl-Coenzyme A Acetyltransferase 2 (Acetoacetyl Coenzyme A Thiolase); Acetyl-Coenzyme A Acetyltransferase 2; Acetyl-CoA Transferase-Like Protein; Cytosolic Acetoacetyl-CoA Thiolase; Acetoacetyl Coenzyme A Thiolase; EC 2.3.1; ACTL
- Gene ID
- 39
- UniProt ID
- Q9BWD1
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys124 and Lys235 (NX_Q9BWD1 [NX_Q9BWD1-1])
Modification sites at PhosphoSitePlus (Q9BWD1)
Glycosylation from GlyGen (Q9BWD1) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001290182.1; NP_005882.2
- Function
- Involved in the biosynthetic pathway of cholesterol.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| VS-0525-XY64 | Anti-ACAT2 Immunohistochemistry Kit | IHC | |
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