Mouse Anti-ACSS1 Recombinant Antibody (VS3-CJ5) (CAT#: VS3-CJ5)

• Immunogen
• Recombinant fragment of human ACSS1 (C-terminus)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human ACSS1

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

• Alternative Names
• ACAS2L; ACECS1; AceCS2L

• Gene ID
• 84532

• UniProt ID
• Q9NUB1

• Sequence Similarities
• Belongs to the ATP-dependent AMP-binding enzyme family.

• Cellular Localization
• Mitochondrion

• Post Translation Modifications
• Reversibly acetylated on Lys-642 (PubMed:16788062).
  The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3.

• Protein Refseq
• NP_001239604.1; NP_001239605.1; NP_001239605.1

• Function
• Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:16788062).
  Acetate is the preferred substrate (PubMed:16788062).
  Can also utilize propionate with a much lower affinity (By similarity).
  Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity).
  Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS