Rabbit Anti-AHSA1 Recombinant Antibody (clone R01-8D9) (CAT#: VS3-FY1642)
The R01-8D9 recombinant monoclonal antibody that specifically targets human AHSA1 protein, a calculated weight of 38 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R01-8D9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/if_selected.jpg
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/ihc_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/ihc_selected.jpg
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6244/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6244/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/4460_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/4460_B_8_5.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/4460_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/4460_A_8_3.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/4460_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/903/4460_A_6_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100591-AHSA1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100591-AHSA1
Specifications
- Immunogen
- Synthetic peptide of human AHA1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat AHSA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-8D9
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 42 kDa.
- Related Disease
- Stomatitis and Cystic Fibrosis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Activator Of HSP90 ATPase Activity 1; C14orf3; AHA1; P38; AHA1, Activator Of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast); AHA1, Activator Of Heat Shock 90kDa Protein ATPase Homolog 1; Activator Of 90 KDa Heat Shock Protein ATPase Homolog 1; Chromosome 14 Open Reading Frame 3; HAha1
- Gene ID
- 10598
- UniProt ID
- O95433
- Sequence Similarities
- Belongs to the AHA1 family.
- Cellular Localization
- Cytosol
- Protein Refseq
- NP_001308370.1; NP_036243.1
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0506MZ | Mouse Anti-AHSA1 Recombinant Antibody (clone CBLS-058) | IHC-P, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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