Rabbit Anti-ALAS1 Recombinant Antibody (clone 2A9)
CAT#: ZG-0541J
This product is a rabbit antibody that recognizes ALAS1.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-ALAS1 Antibody (ZG-0541J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: ALAS1 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 71, 13 kDa
Observed band size: 71 kDa
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/407_F8_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17498/if_selected.jpg
Normal Tissue
Figure 3 Adrenal gland
High expression in adrenal gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/81753_B_4_5_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17498/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/81753_A_7_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/81753_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/81753_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/81753_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35860/81753_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000023330-ALAS1
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Specifications
- Immunogen
- A synthesized peptide derived from human Alas1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ALAS1
- Species Reactivity
- Human
- Clone
- 2A9
- Applications
- ELISA, WB
- Conjugate
- Uconjugated
- Related Disease
- Cardiovascular
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot.
WB:1:500-1:5000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ALAS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0577MZ | Mouse Anti-ALAS1 Recombinant Antibody (clone CBLS-089) | WB | Mouse IgG2a |
| MRO-0046-CN | Rabbit Anti-ALAS1 Recombinant Antibody (clone CBACN-018) | WB, IF | Rabbit IgG |
| VS3-CJ627 | Rabbit Anti-ALAS1 Recombinant Antibody (VS3-CJ627) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0130 | Hi-Affi™ Rabbit Anti-ALAS1 Recombinant Antibody (clone DS130AB) | WB, IF | Rabbit IgG |
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