Rabbit Anti-ALDH1A1 Antibody (VS-0924-YC20) - Cancer Stem Cell Marker (CAT#: VS-0924-YC20)
This product is an anti-ALDH1A1 antibody (VS-0924-YC20). Targeting human ALDH1A1 protein, a recognized marker for cancer stem cells (CSCs), this antibody can detect ALDH1A1 in CSC tissues or cells. It is applicable for Immunohistochemistry at 1/2500-1/5000 dilutions, Western blot at 0.1-0.5 µg/mL, and Immunofluorescence at 0.5-2 µg/mL.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/1584_G10_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/1584_G10_1_selected.jpg
Figure 2 High expression in epididymis
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/8966_A_1_6_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/8966_A_1_6_val_selected.jpg
Figure 3 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_7_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_6_6.jpg
Figure 5 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_3_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_3_1.jpg
Figure 6 RNA cell line category: Cell line enhanced (A549, Hep G2, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165092-ALDH1A1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165092-ALDH1A1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ALDH1A1
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/2500-1/5000
Western Blot (WB): 0.1-0.5 µg/mL
Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- ALDH1; PUMB1; RALDH1
- Gene ID
- 216
- UniProt ID
- P00352
- Long Name
- Aldehyde dehydrogenase 1 family, member A1
- Cellular Localization
- Cell projection, Cytoplasm
- Function
- Inhibited by citral, disulfiram, and cyanamide. Activated by diethylstilbestrol (PubMed:19296407).
Inhibited by duocarmycin analogs (PubMed:26373694).
Recommended Products
Isotype Control
Secondary Antibody
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0567MZ | Mouse Anti-ALDH1A1 Recombinant Antibody (clone nBcdbn 72304) | WB | Mouse IgG |
| MRO-1634-CN | Rabbit Anti-ALDH1A1 Polyclonal Antibody (MRO-1634-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1635-CN | Rabbit Anti-ALDH1A1 Polyclonal Antibody (MRO-1635-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0279F | Mouse Anti-ALDH1A1 Recombinant Antibody (ZG-0279F) | WB, IHC, ELISA | Mouse IgG |
| VS7-HM77 | Mouse Anti-ALDH1A1 Recombinant Antibody (clone 2B2G1) | WB, IHC, FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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