Rabbit Anti-ALDH1A1 Antibody (VS-0924-YC20) - Cancer Stem Cell Marker
CAT#: VS-0924-YC20
This product is an anti-ALDH1A1 antibody (VS-0924-YC20). Targeting human ALDH1A1 protein, a recognized marker for cancer stem cells (CSCs), this antibody can detect ALDH1A1 in CSC tissues or cells. It is applicable for Immunohistochemistry at 1/2500-1/5000 dilutions, Western blot at 0.1-0.5 µg/mL, and Immunofluorescence at 0.5-2 µg/mL.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/1584_G10_1_selected.jpg
Normal Tissue
Figure 2 High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2123/8966_A_1_6_val_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_6_6.jpg
Normal Tissue
Figure 5 Stomach
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20690/47870_A_3_1.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A549, Hep G2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165092-ALDH1A1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ALDH1A1
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/2500-1/5000
Western Blot (WB): 0.1-0.5 µg/mL
Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- ALDH1; PUMB1; RALDH1
- Gene ID
- 216
- UniProt ID
- P00352
- Long Name
- Aldehyde dehydrogenase 1 family, member A1
- Cellular Localization
- Cell projection, Cytoplasm
- Function
- Inhibited by citral, disulfiram, and cyanamide. Activated by diethylstilbestrol (PubMed:19296407).
Inhibited by duocarmycin analogs (PubMed:26373694).
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ALDH1A1"
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|---|---|---|---|
| MOR-0136 | Hi-Affi™ Rabbit Anti-ALDH1A1 Recombinant Antibody (clone DS136AB) | WB, IHC-P | Rabbit IgG |
| MOR-0063-FY | Rabbit Anti-ALDH1A1 Recombinant Antibody (clone AFY0034) | IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-1634-CN | Rabbit Anti-ALDH1A1 Polyclonal Antibody (MRO-1634-CN) | WB, IF, IHC, FC | Rabbit IgG |
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| VS7-HM77 | Mouse Anti-ALDH1A1 Recombinant Antibody (clone 2B2G1) | WB, IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0325-XY87 | Anti-ALDH1A1 Immunohistochemistry Kit | IHC | |
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