Rabbit Anti-ANP32B Recombinant Antibody (clone R03-6H2) (CAT#: VS3-FY1830)
Clone R03-6H2 is a rabbit recombinant monoclonal antibody that has been developed to detect human ANP32B. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The binding specificity of the PHAPI2 antibody was determined using western blot analysis of PHAPI2 in K562, C6 lysates and immunohistochemistry analysis of paraffin-embedded human colon cancer tissue.
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WB
Figure 1 Anti-ANP32B Antibody (VS3-FY1830) in WB.
The PHAPI2 expression in K562, C6 lysates were detected using the anti-PHAPI2 by western blot assay.
IHC
Figure 2 Anti-ANP32B Antibody (VS3-FY1830) in IHC.
Immunohistochemistry staining of paraffin-embedded human colon cancer tissue using the anti-PHAPI2/APRIL antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PHAPI2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ANP32B
- Species Reactivity
- Human, Rat
- Clone
- R03-6H2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 29 kDa, while the observed molecular weight stands at 29 kDa.
- Related Disease
- Human colon cancer tissue
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Acidic Nuclear Phosphoprotein 32 Family Member B; Acidic Protein Rich In Leucines; Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member B; Putative HLA-DR-Associated Protein I-2; Silver-Stainable Protein SSP29; PHAPI2; APRIL; Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B; SSP29
- Gene ID
- 10541
- UniProt ID
- Q92688
- Sequence Similarities
- Belongs to the ANP32 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Some glutamate residues are glycylated by TTLL8.
This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group ().
(Q92688-AN32B_HUMAN)
Directly cleaved by caspase-3/CASP3. (Q92688-AN32B_HUMAN)
Ubiquitination at Lys86 and Lys99 (NX_Q92688 [NX_Q92688-1])
Modification sites at PhosphoSitePlus (Q92688)
Glycosylation from GlyGen (Q92688) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_006392.1
- Function
- As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis.
Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes.
Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0178 | Hi-Affi™ Rabbit Anti-ANP32B Recombinant Antibody (clone DS178AB) | WB, IHC-P, IF, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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