AbPlus™ Anti-ANPEP Magnetic Beads (CBACN-106)
CAT#: VS-0424-XY13
The AbPlus Anti-ANPEP Magnetic Beads (CBACN-106) is an innovative affinity resin which is bound with anti-ANPEP specific antibody (CBACN-106). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ANPEP protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4625/1612_A1_3_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_4.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_5_4.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HMC-1, HSkMC, HUVEC TERT2, TIME, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166825-ANPEP
❮
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-ANPEP specific antibody (CBACN-106)
- Target
- ANPEP
- Immunogen
- Recombinant human ANPEP protein (aa 1-260)
- Target Species
- Human, Mouse, Rat
- Antibody Clone
- CBACN-106
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.
- Alternative Names
- Alanyl Aminopeptidase, Membrane; Microsomal Aminopeptidase; Aminopeptidase M; Myeloid Plasma Membrane Glycoprotein CD13; Alanyl (Membrane) Aminopeptidase; Membrane Alanyl Aminopeptidase; Aminopeptidase N; EC 3.4.11.2; GP150; CD13; PEPN
- Gene ID
- 290
- UniProt ID
- P15144
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| MOB-69-F(E) | Recombinant Anti-human ANPEP Antibody Fab Fragment | WB, IP, FuncS | Fab |
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| CAT | Product Name | Application | Type |
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| MOB-69-S(P) | Recombinant Anti-human ANPEP Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| MRO-0272-CN | Rabbit Anti-ANPEP Recombinant Antibody (clone CBACN-106) | WB, IF, IHC, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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