Mouse Anti-NHP ANPEP Recombinant Antibody (clone WM15) (CAT#: VS-1024-XY75)
This monoclonal antibody of mouse origin is developed to specifically target ANPEP, with demonstrated reactivity against human and non-human primate species. The antibody is generated using human AML cells as the immunogen, ensuring exceptional binding affinity. Optimized for flow cytometry (FC) applications, it provides reliable and precise detection of ANPEP expression. Its high specificity and sensitivity make it an invaluable resource for researchers exploring ANPEP-related pathways in both normal and diseased states.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4625/1612_A1_3_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4625/1612_A1_3_selected.jpg
Figure 2 Colon
(Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_3.jpg
(Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_3.jpg
Figure 3 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_4.jpg
Figure 4 Gallbladder
(Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_5_4.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_5_4.jpg
Figure 5 Kidney
(Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_5.jpg
(Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HMC-1, HSkMC, HUVEC TERT2, TIME, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166825-ANPEP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166825-ANPEP
Specifications
- Immunogen
- Human AML cells
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ANPEP
- Species Reactivity
- Human, Non-human primate
- Clone
- WM15
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 2-3 µg/mL
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-69-F(E) | Recombinant Anti-human ANPEP Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-69-F(E) | Recombinant Human Anti-human ANPEP Antibody Fab Fragment | WB, FC, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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