Mouse Anti-ANXA4 Recombinant Antibody (clone SAIC-14C-10F12) (CAT#: FAMAB-1012CQ)
This product is a recombinant mouse antibody that recognizes ANXA4. The antibody was purified by affinity chromatography. It can be used in WB, Immuno-MRM, ELISA.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 ELISA data from screening recombinant monoclonal antibodies using recombinant proteins or overexpressed lysates.
ELISA data were reduced using Prism software to calculate the 50% binding point (B50%). The B50% values should not be interpreted as absolute numbers that define mAb performance (i.e., not analogously to affinity constants); instead, the B50% values can be used as relative values to compare the performances of the mAb within this group of mAbs.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
WB
Figure 2 Images of Western blots from screening the recombinant and hybridoma monoclonal antibodies (mAbs) using recombinant proteins, overexpressed lysates, and 6 breast cancer NCI60 cell line lysates.
The purified mAbs were run as controls alongside the recombinant proteins or the overexpressed lysate-proteins. Because the mAbs' protein targets (except for TSHB and CGB) were expected to be detected in breast cancer cell lines. For the Western blots using the NCI60 breast cancer cell lines.
Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.
Specifications
- Immunogen
- Peptide DEGNYLDDALVR conjugated to KLH.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ANXA4
- Species Reactivity
- Human
- Clone
- SAIC-14C-10F12
- Applications
- WB, Immuno-MRM, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Annexin A4; Carbohydrate-Binding Protein P33/P41; Placental Anticoagulant Protein II; 35-Beta Calcimedin; Chromobindin-4; Lipocortin IV; Endonexin I; Protein II; Annexin-4; PAP-II; P32.5
- Gene ID
- 307
- UniProt ID
- P09525
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1621z | Mouse Anti-ANXA4 Recombinant Antibody (clone 2A5) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-0623MZ | Mouse Anti-ANXA4 Recombinant Antibody (clone 2E4) | ELISA, WB | Mouse IgG2a |
| MRO-0100-CN | Rabbit Anti-ANXA4 Recombinant Antibody (clone JG37-83) | WB, IF, IHC, FC | Rabbit IgG |
| VS-0923-FY16 | Recombinant Mouse Anti-ANXA4 Antibody (VS-0923-FY16) | ELISA, WB | Mouse IgG1 |
| VS3-FY1970 | Rabbit Anti-ANXA4 Recombinant Antibody (clone R04-3H8) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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