Rabbit Anti-ARAF Recombinant Antibody (clone R02-0C6) (CAT#: VS3-FY1962)
This rabbit recombinant monoclonal antibody shows positive verification in western blot analysis of A RAF in Hela, Ramos lysates. It is specifically engineered to detect human ARAF. It is also suitable for use in a variety of assays, including WB, IHC-P. This R02-0C6 antibody is validated for western blot analysis of A RAF in Hela, Ramos lysates.
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WB
Figure 1 Anti-ARAF Antibody (VS3-FY1962) in WB.
The ARAF expression in Hela, Ramos lysates were detected using the anti-ARAF by western blot assay.
Specifications
- Immunogen
- Recombinant human A RAF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ARAF
- Species Reactivity
- Human
- Clone
- R02-0C6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 68 kDa, while the observed molecular weight stands at 68 kDa.
- Related Disease
- Pallister-Killian Syndrome and Skin Melanoma. Among its related pathways are Prolactin Signaling and Signaling downstream of RAS mutants
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- A-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; ARAF1; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase
- Gene ID
- 369
- UniProt ID
- P10398
- Sequence Similarities
- Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P10398)
Modification sites at neXtProt (NX_P10398)
Glycosylation from GlyGen (P10398) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001243125.1; NP_001243126.1; NP_001645.1
- Function
- Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
MAPK Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0408MZ | Mouse Anti-ARAF Recombinant Antibody (clone CBLS-012) | WB | Mouse IgG2a |
| MRO-0115-CN | Rabbit Anti-ARAF Recombinant Antibody (clone CBACN-039) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ561 | Rabbit Anti-ARAF Recombinant Antibody (VS3-CJ561) | WB, ICC, IF, IHC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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