CytoStream™ Rabbit Anti-ARAF Recombinant Antibody (VS13-YC63)
CAT#: VS13-YC63
The CytoStream™ Rabbit Anti-ARAF Recombinant Antibody is specific for human and mouse ARAF. ARAF, a proto-oncogene from the RAF subfamily, may be involved in cell growth and development, with different isoforms arising from alternative splicing. The anti-ARAF antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_7_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66326/154072_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078061-ARAF
❮
❯
Specifications
- Immunogen
- Recombinant human A-RAF, aa 146-310.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ARAF
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Pallister-Killian Syndrome and Skin Melanoma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- A-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; ARAF1; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase;
- Gene ID
- 369
- UniProt ID
- P10398
- Long Name
- A-Raf Proto-Oncogene, Serine/Threonine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
- Cellular Localization
- Cytosol
- Protein Refseq
- NP_001243125.1; NP_001243126.1; NP_001645.1
- Function
- Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS13-YC63. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ARAF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0408MZ | Mouse Anti-ARAF Recombinant Antibody (clone CBLS-012) | WB | Mouse IgG2a |
| MRO-0115-CN | Rabbit Anti-ARAF Recombinant Antibody (clone CBACN-039) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ561 | Rabbit Anti-ARAF Recombinant Antibody (VS3-CJ561) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1962 | Rabbit Anti-ARAF Recombinant Antibody (clone R02-0C6) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY488 | Anti-ARAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY489 | Anti-Mouse ARAF Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
-2.jpg)
Application: IB, ELISA, FC, FuncS
-CB2006C17L-4.jpg)
Application: WB, ELISA
Application: ELISA, FC, WB, FuncS
Application: WB, ELISA, IF, FuncS, Apop, BA
Application: FC, IHC, Cyt, FuncS
Application: ELISA, Block, WB, FC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
