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  • Rabbit Anti-ARHGAP25 Recombinant Antibody (clone R07-6G6)

Rabbit Anti-ARHGAP25 Recombinant Antibody (clone R07-6G6) (CAT#: VS3-FY1654)

Clone R07-6G6 is a recombinant monoclonal antibody that has been developed to detect human ARHGAP25 protein, a calculated weight of 73 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R07-6G6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100).


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Specific Inquiry
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61395/1419_G8_3_selected.jpg
Normal Tissue
Figure 2 Spleen
Figure 2 Spleen
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70561_B_9_4_val_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70561_B_9_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_9_3.jpg
Figure 5 Testis
Figure 5 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_4_6.jpg
Figure 6 Lymph node
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, NB-4, U-266/70)
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, NB-4, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163219-ARHGAP25

Specifications

  • Immunogen
  • Recombinant human ARHGAP25 protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat ARHGAP25
  • Species Reactivity
  • Human, Rat
  • Clone
  • R07-6G6
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • MW
  • The calculated weight of the target protein is approximately 73 kDa, while the observed molecular weight stands at 73 kDa.
  • Related Disease
  • Rhabdomyosarcoma 2

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100

Target

  • Alternative Names
  • Rho GTPase Activating Protein 25; Rho-Type GTPase-Activating Protein 25; Epididymis Secretory Protein Li 308; Rho GTPase-Activating Protein 25; HEL-S-308; KAIA0053; KIAA0053

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ARHGAP25"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0240 Hi-Affi™ Rabbit Anti-ARHGAP25 Recombinant Antibody (clone DS240AB) WB, IP, IF Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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