Rabbit Anti-ARHGAP25 Recombinant Antibody (clone R07-6G6)
CAT#: VS3-FY1654
Clone R07-6G6 is a recombinant monoclonal antibody that has been developed to detect human ARHGAP25 protein, a calculated weight of 73 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R07-6G6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61395/1419_G8_3_selected.jpg
Normal Tissue
Figure 2 Spleen
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70561_B_9_4_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70561_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35346/70253_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEL, HMC-1, NB-4, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163219-ARHGAP25
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Specifications
- Immunogen
- Recombinant human ARHGAP25 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ARHGAP25
- Species Reactivity
- Human, Rat
- Clone
- R07-6G6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 73 kDa, while the observed molecular weight stands at 73 kDa.
- Related Disease
- Rhabdomyosarcoma 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Rho GTPase Activating Protein 25; Rho-Type GTPase-Activating Protein 25; Epididymis Secretory Protein Li 308; Rho GTPase-Activating Protein 25; HEL-S-308; KAIA0053; KIAA0053
- Gene ID
- 9938
- UniProt ID
- P42331
- Protein Refseq
- XP_011531509.1; XP_011531511.1; XP_016860915.1; XP_011531512.1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ARHGAP25"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0240 | Hi-Affi™ Rabbit Anti-ARHGAP25 Recombinant Antibody (clone DS240AB) | WB, IP, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY502 | Anti-ARHGAP25 Immunohistochemistry Kit | IHC | |
| VS-0525-XY503 | Anti-Mouse ARHGAP25 Immunohistochemistry Kit | IHC |
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