CytoStream™ Rabbit Anti-ATAD2 Recombinant Antibody (VS13-YC72) (CAT#: VS13-YC72)
The CytoStream™ Rabbit Anti-ATAD2 Recombinant Antibody is specific for human ATAD2. ATAD2 encodes an ATPase associated with cellular activities and protein complex assembly, possessing two AAA domains and a bromodomain, performing chaperone-like functions. The anti-ATAD2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29424/602_G5_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29424/602_G5_1_red_green.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29424/583_G5_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29424/583_G5_1_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29424/598_G5_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29424/598_G5_1_red_green.jpg
Figure 4 Esophagus
(Squamous epithelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_B_6_1.jpg
(Squamous epithelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_B_6_1.jpg
Figure 5 Colon
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_7_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_7_5.jpg
Figure 7 Testis
(Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_6_6.jpg
(Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_7_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56158/121376_A_7_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156802-ATAD2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156802-ATAD2
Specifications
- Immunogen
- Recombinant human ATAD2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATAD2
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- ATPase Family, AAA Domain Containing 2; AAA Nuclear Coregulator Cancer-Associated Protein; ANCCA; ATPase Family AAA Domain-Containing Protein 2; EC 3.6.1.3; PRO2000; CT137;
- Gene ID
- 29028
- UniProt ID
- Q6PL18
- Long Name
- ATPase Family, AAA Domain Containing 2
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-224 | Human Anti-ATAD2 Recombinant Antibody (clone D372) | ELISA, IP-MS | Human IgG1 |
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