Rabbit Anti-ATG10 Recombinant Antibody (clone R02-0B2)
CAT#: VS3-FY1973
The antibody detects western blot analysis of ATG10 in Hela, A549, U251 lysates. It is a amazing rabbit recombinant monoclonal antibody that is engineered to detect human ATG10. It is suitable for use in a variety of assays, including WB, IP. The R02-0B2 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/143872_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/143872_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/143872_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/143872_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/143872_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44163/143872_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152348-ATG10
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WB
Figure 1 Anti-ATG10 Antibody (VS3-FY1973) in WB.
The ATG10 expression in Hela, A549, U251 lysates were detected using the anti-ATG10 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human ATG10
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATG10
- Species Reactivity
- Human
- Clone
- R02-0B2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Neurodegeneration With Brain Iron Accumulation
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Autophagy Related 10; Autophagy-Related Protein 10; APG10L; ATG10 Autophagy Related 10 Homolog (S. Cerevisiae); APG10 Autophagy 10-Like (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG10; ATG10 Autophagy Related 10 Homolog
- Gene ID
- 83734
- UniProt ID
- Q9H0Y0
- Sequence Similarities
- Belongs to the ATG10 family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9H0Y0)
Modification sites at neXtProt (NX_Q9H0Y0)
- Protein Refseq
- XP_005248667.1; XP_005248668.1; XP_005248669.1; XP_011541962.1; XP_011541963.1
- Function
- E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity).
Plays a role in adenovirus-mediated cell lysis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ATG10"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0277 | Hi-Affi™ Rabbit Anti-ATG10 Recombinant Antibody (clone DS277AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-HM149 | Mouse Anti-ATG10 Recombinant Antibody (clone 8A9B3) | WB, FCM | Mouse IgG1 |
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