Rabbit Anti-ATG10 Recombinant Antibody (clone R02-0B2) (CAT#: VS3-FY1973)
The antibody detects western blot analysis of ATG10 in Hela, A549, U251 lysates. It is a amazing rabbit recombinant monoclonal antibody that is engineered to detect human ATG10. It is suitable for use in a variety of assays, including WB, IP. The R02-0B2 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunoprecipitation (dilution 1/20).
Specific Inquiry
WB
Figure 1 Anti-ATG10 Antibody (VS3-FY1973) in WB.
The ATG10 expression in Hela, A549, U251 lysates were detected using the anti-ATG10 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human ATG10
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATG10
- Species Reactivity
- Human
- Clone
- R02-0B2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Neurodegeneration With Brain Iron Accumulation
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Autophagy Related 10; Autophagy-Related Protein 10; APG10L; ATG10 Autophagy Related 10 Homolog (S. Cerevisiae); APG10 Autophagy 10-Like (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG10; ATG10 Autophagy Related 10 Homolog
- Gene ID
- 83734
- UniProt ID
- Q9H0Y0
- Sequence Similarities
- Belongs to the ATG10 family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9H0Y0)
Modification sites at neXtProt (NX_Q9H0Y0)
- Protein Refseq
- XP_005248667.1; XP_005248668.1; XP_005248669.1; XP_011541962.1; XP_011541963.1
- Function
- E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity).
Plays a role in adenovirus-mediated cell lysis.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0277 | Hi-Affi™ Rabbit Anti-ATG10 Recombinant Antibody (clone DS277AB) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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