Rabbit Anti-ATG16L1 Recombinant Antibody (clone R05-8U2) (CAT#: VS3-XY3269)
This is a recombinant rabbit antibody that specifically recognizes human ATG16L1 (Autophagy related 16 like 1), which plays an essential role in both canonical and non-canonical autophagy. It also has cross-reactivity against mouse or rat ATG16L1. It has been reported for use in Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12577/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12577/if_selected.jpg
Figure 2 IHC staining of human gallbladder
(Immunohistochemical staining of human gallbladder shows membrane positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_4_4_selected.jpg
(Immunohistochemical staining of human gallbladder shows membrane positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_4_4_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_9_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_9_4.jpg
Figure 6 Kidney
(Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_8_5.jpg
(Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Low Intensity: Weak Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Low Intensity: Weak Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63900/151700_A_4_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085978-ATG16L1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085978-ATG16L1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ATG16L1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-8U2
- Applications
- WB, IHC-P
- Related Disease
- Inflammatory Bowel Disease 10; Inflammatory Bowel Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- IBD10; WDR30; APG16L; ATG16A; ATG16L
- Gene ID
- 55054
- UniProt ID
- Q676U5
- Long Name
- Autophagy related 16 like 1
- Cellular Localization
- Cytoplasm, Endosome, Lysosome, Membrane
- Post Translation Modifications
- Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
- Protein Refseq
- XP_005246143.1; XP_005246141.1
- Function
- Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins.
Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling.
Plays a role in regulating morphology and function of Paneth cell.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "ATG16L1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3228z | Mouse Anti-ATG16L1 Recombinant Antibody (clone 41G9) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MRO-0136-CN | Rabbit Anti-ATG16L1 Recombinant Antibody (clone JU30-62) | WB | Rabbit IgG |
| VS3-QX71 | Mouse Anti-ATG16L1 Recombinant Antibody (clone 54CT27.2.6) | WB, IHC | Mouse IgG1 |
| VS3-WK805 | Rabbit Anti-ATG16L1 Recombinant Antibody (clone VS3-WK805) | WB, ELISA | Rabbit IgG |
| VS3-FY107 | Recombinant Rabbit Anti-ATG16L1 Antibody (clone R03-4G8) | WB | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4493 | Hi-Affi™ Rabbit Anti-ATG16L1 Recombinant Antibody (clone TH1DS) | WB, IF, ICC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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