Rabbit Anti-ATP1B3 Recombinant Antibody (clone R02-1B4)
CAT#: VS3-FY1658
Clone R02-1B4 is a rabbit recombinant antibody that recognizes human ATP1B3 protein, a calculated weight of 32 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R02-1B4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48963/855_B11_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48963/ihc_selected.jpg
Normal Tissue
Figure 3 Adrenal gland
High expression in adrenal gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_B_5_5_selected.jpg
Normal Tissue
Figure 4 Endometrium
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_B_5_3.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000069849-ATP1B3
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Specifications
- Immunogen
- Synthetic peptide of human ATP1B3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ATP1B3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-1B4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 32 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Newfoundland Rod-Cone Dystrophy and Petroclival Meningioma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- ATPase Na+/K+ Transporting Subunit Beta 3; Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic); Sodium/Potassium-Transporting ATPase Subunit Beta-3; Sodium/Potassium-Dependent ATPase Subunit Beta-3; ATPase, Na+/K+ Transporting, Beta 3 Polypeptide; Sodium Pump Subunit Beta-3
- Gene ID
- 483
- UniProt ID
- P54709
- Sequence Similarities
- Belongs to the X(+)/potassium ATPases subunit beta family.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Disulfide bond, Glycoprotein
- Protein Refseq
- NP_001670.1
- Function
- This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na⁺ and K⁺ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ATP1B3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0292 | Hi-Affi™ Rabbit Anti-ATP1B3 Recombinant Antibody (clone DS292AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY201 | Anti-ATP1B3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY609 | Anti-Human ATP1B3 Immunohistochemistry Kit | IHC |
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