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  • Rabbit Anti-ATP1B3 Recombinant Antibody (clone R02-1B4)

Rabbit Anti-ATP1B3 Recombinant Antibody (clone R02-1B4) (CAT#: VS3-FY1658)

Clone R02-1B4 is a rabbit recombinant antibody that recognizes human ATP1B3 protein, a calculated weight of 32 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R02-1B4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).


See more Engineered Antibody products for ATP1B3
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48963/855_B11_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48963/ihc_selected.jpg
Figure 3 Adrenal gland
Figure 3 Adrenal gland
(High expression in adrenal gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_B_5_5_selected.jpg
Figure 4 Endometrium
Figure 4 Endometrium
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_B_5_3.jpg
Figure 5 Colon
Figure 5 Colon
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_A_9_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20697/156792_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000069849-ATP1B3

Specifications

  • Immunogen
  • Synthetic peptide of human ATP1B3
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ATP1B3
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R02-1B4
  • Applications
  • Western Blot
  • MW
  • The calculated weight of the target protein is approximately 32 kDa, while the observed molecular weight stands at 40 kDa.
  • Related Disease
  • Newfoundland Rod-Cone Dystrophy and Petroclival Meningioma

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000

Target

  • Alternative Names
  • ATPase Na+/K+ Transporting Subunit Beta 3; Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic); Sodium/Potassium-Transporting ATPase Subunit Beta-3; Sodium/Potassium-Dependent ATPase Subunit Beta-3; ATPase, Na+/K+ Transporting, Beta 3 Polypeptide; Sodium Pump Subunit Beta-3
  • Sequence Similarities
  • Belongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular Localization
  • Cell membrane, Membrane
  • Post Translation Modifications
  • Disulfide bond, Glycoprotein
  • Function
  • This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na⁺ and K⁺ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.

Recommended Products

Isotype Control

Secondary Antibody

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ATP1B3"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0292 Hi-Affi™ Rabbit Anti-ATP1B3 Recombinant Antibody (clone DS292AB) WB, IHC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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