Mouse Anti-b96.11 Recombinant Antibody (clone 8E6G4) (CAT#: FAMAB-0380CQ)

Recombinant Mouse Antibody clone 8E6G4, which is specific to b96.11. The monoclonal anti-Id MAb 8E6G4 specifically targeting human monoclonal GAD65 Ab b96.11. 8E6G4 binds to human GAD65Ab b96.11 with high specificity and affinity and 8E6G4 binding to GAD65Ab b96.11 is specifically blocked by GAD65.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
DB

Figure 1 MAb 8E6G4 binds specifically to b96.11.

Figure 1 MAb 8E6G4 binds specifically to b96.11.

Purified MAb 8E6G4, supernatant of hybridoma 8E6G4, or BSA were analyzed for binding by b96.11 by Dot blot. From left: supernatant of hybridoma 8E6G4, purified MAb 8E6G4, and BSA.

Wang, X., Zhang, A., Liu, Y., Chen, S., Feng, Z., Shang, W., ... & Hampe, C. S. (2012). Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PloS one, 7(2), e32515.

IP, WB

Figure 2 MAb 8E6G4 binds specifically to b96.11.

Figure 2 MAb 8E6G4 binds specifically to b96.11.

Binding of MAb 8E6G4 and supernatant of hybridoma 8E6G4 to b96.11 was analyzed by immunoprecipitation followed by Western blot analysis. Binding of MAb 8E6G4 to control antibody hLF served as negative control. From left: supernatant of hybridoma 8E6G4 with b96.11, MAb 8E6G4 with b96.11, and MAb 8E6G4 with hLF.

Wang, X., Zhang, A., Liu, Y., Chen, S., Feng, Z., Shang, W., ... & Hampe, C. S. (2012). Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PloS one, 7(2), e32515.

ELISA

Figure 3 MAb 8E6G4 binds specifically to b96.11.

Figure 3 MAb 8E6G4 binds specifically to b96.11.

MAb 8E6G4 binds to b96.11 with high affinity. Dissociation constant was calculated by non-competing ELISA. Different concentrations of b96.11 were tested for binding by MAb 8E6G4. Kd of each concentration was calculated. The Kd was calculated as follows: Kd = 2(nKd′-Kd)/(n-1), where n = [b96.11]′/[b96.11]. The average Kd calculated was 480.4 pM.

Wang, X., Zhang, A., Liu, Y., Chen, S., Feng, Z., Shang, W., ... & Hampe, C. S. (2012). Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PloS one, 7(2), e32515.

ELISA

Figure 4 MAb 8E6G4 competes with b96.11 for binding to GAD65.

Figure 4 MAb 8E6G4 competes with b96.11 for binding to GAD65.

A: GAD65 was translated in vitro using rabbit reticulocyte lysate. Different amounts (0–40 µl, corresponding to 0–160 ng) of recombinant GAD65 were incubated with 1.4 µg/ml MAb 8E6G4 and subsequently analyzed for binding to b96.11 by ELISA. Rabbit reticulocyte lysate alone was used as a negative control (open squares). Binding in the absence of competitor was set as 100%. B: Binding of monoclonal GAD65Ab b96.11 (black squares), b78 (open squares), and N-GAD65mAb (open circles) to radiolabeled GAD65 in the presence of the indicated concentrations of MAb 8E6G4 was determined. Binding is reported at percent binding, binding of the respective antibody to radiolabeled GAD65 in the absence of MAb 8E6G4 is set as 100%.

Wang, X., Zhang, A., Liu, Y., Chen, S., Feng, Z., Shang, W., ... & Hampe, C. S. (2012). Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PloS one, 7(2), e32515.

FuncS

Figure 5 Cumulative incidence rate of diabetes development.

Figure 5 Cumulative incidence rate of diabetes development.

NOD mice were injected with 50 and 100 µg MAb 8E6G4, presented as black circles and squares, respectively. Control animals were injected with PBS (white circles) or control mouse monoclonal antibody (white squares). Animals were weekly monitored for diabetes development. Cumulative incidence rate of diabetes development is presented as percentage.

Wang, X., Zhang, A., Liu, Y., Chen, S., Feng, Z., Shang, W., ... & Hampe, C. S. (2012). Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PloS one, 7(2), e32515.

FuncS

Figure 6 The histopathology of pancreatic islets at onset of diabetes or at 40 weeks of age.

Figure 6 The histopathology of pancreatic islets at onset of diabetes or at 40 weeks of age.

Representative images of pancreatic islets from animals injected with 50 µg (A) and 100 µg (B) MAb 8E6G4 IgG, or control animals (C). Pancreatic tissues were sectioned and stained with hematoxylin and eosin.

Wang, X., Zhang, A., Liu, Y., Chen, S., Feng, Z., Shang, W., ... & Hampe, C. S. (2012). Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PloS one, 7(2), e32515.


Specifications

  • Immunogen
  • b96.11
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • b96.11
  • Clone
  • 8E6G4
  • Applications
  • ELISA, IP, IHC, Dot, WB, FuncS
  • Related Disease
  • Type 1 diabetes

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Dot Blot, Immunoprecipitation, Western Blot and Functional Assay. Please see the Published Data for further information.

Target

  • Alternative Names
  • b96.11; Anti-GAD65 antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 8E6G4"

See other products for "b96.11"

Fab Fragment Antibody

CAT Product Name Application Type
FAMAB-0380CQ-F(E) Mouse Anti-b96.11 Recombinant Antibody (clone 8E6G4); Fab Fragment ELISA, IP, IHC, DB Mouse Fab

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0380CQ-S(P) Mouse Anti-b96.11 Recombinant Antibody (clone 8E6G4); scFv Fragment ELISA, IP, IHC, DB Mouse scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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