Rabbit Anti-BAG2 Recombinant Antibody (clone R04-4A5) (CAT#: VS3-XY3273)
The antibody is a recombinant rabbit antibody against BAG cochaperone 2 (BAG2), which acts as a nucleotide-exchange factor (NEF) promoting the release of ADP. It specifically reacts with human, mouse and rat BAG2. It has been reported for use in Western Blot, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18862/175_C9_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18862/175_C9_1_selected.jpg
Figure 2 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_4_6.jpg
Figure 3 Smooth muscle
(Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_B_5_7.jpg
(Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_B_5_7.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_9_8.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112208-BAG2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112208-BAG2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BAG2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4A5
- Applications
- WB, ICC, IF
- Related Disease
- Chronic Frontal Sinusitis; Cystic Fibrosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- BAG-2; dJ417I1.2
- Gene ID
- 9532
- UniProt ID
- O95816
- Long Name
- BAG cochaperone 2
- Cellular Localization
- Axon, Cytoxol
- Post Translation Modifications
- Ubiquitination at Lys5, Lys123, Lys154, and Lys200 (NX_O95816 [NX_O95816-1])
Modification sites at PhosphoSitePlus (O95816)
Glycosylation from GlyGen (O95816) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011513301.1; XP_005249547.1
- Function
- Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
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