AbPlus™ Anti-BRD3 Magnetic Beads (VS-0724-YC857) (CAT#: VS-0724-YC857)
The AbPlus Anti-BRD3 Magnetic Beads (VS-0724-YC857) is an innovative affinity resin which is bound with anti-BRD3 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of BRD3 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/if_selected.jpg

Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows nuclear positivity in a subset of cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_8_4_selected.jpg
(Immunohistochemical staining of human spleen shows nuclear positivity in a subset of cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_8_4_selected.jpg

Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/787_A5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/787_A5_3_blue_red_green.jpg

Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/782_A5_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/782_A5_1_blue_red_green.jpg

Figure 5 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_9_5.jpg
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_9_5.jpg

Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_3.jpg

Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_4.jpg

Figure 8 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_7_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_7_5.jpg

Figure 9 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_4_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_4_6.jpg

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169925-BRD3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169925-BRD3
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-BRD3 specific antibody
- Target
- BRD3
- Immunogen
- Recombinant human BRD3 fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008]
- Alternative Names
- Bromodomain Containing 3; RING3-Like Protein; RING3L; Bromodomain-Containing Protein 3 Short Isoform; Bromodomain-Containing Protein 3; Bromodomain-Containing 3; RING3-Like; KIAA0043; ORFX;
- Gene ID
- 8019
- UniProt ID
- Q15059
Downloads
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Please select product type
IgG Antibody Products
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-250 | Human Anti-BRD3 Recombinant Antibody (clone D385) | ELISA, IP-MS | Human IgG1 |
MOB-1485MZ | Recombinant Mouse Anti-Human BRD3 Antibody | IHC-P, WB | Mouse antibody |
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