Human Anti-BRD3 Recombinant Antibody (clone 2506W1343) (CAT#: VS-0625-FY1448)
This recombinant antibody is a human IgG1 antibody, developed through recombinant antibody (rAb) phage display. Its specificity and affinity for the BRD3 protein have been rigorously tested under non-denaturing conditions. While no specific applications are detailed, this antibody is well-suited for recognizing BRD3 in its native state. The antibody's epitope is located within amino acids 306-416 of the BRD3 protein, providing a precise tool for research and potential custom assay development.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/if_selected.jpg
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows nuclear positivity in a subset of cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_8_4_selected.jpg
(Immunohistochemical staining of human spleen shows nuclear positivity in a subset of cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_8_4_selected.jpg
Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/787_A5_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/787_A5_3_blue_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/782_A5_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/782_A5_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_9_5.jpg
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_9_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_7_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_7_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_4_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51830/120386_A_4_6.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169925-BRD3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169925-BRD3
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The antibody reacts with human BRD3.
- Species Reactivity
- Human
- Clone
- 2506W1343
- Applications
- ELISA, Immunoprecipitation Mass Spectrometry
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (over 6 months). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Optimal dilutions for assays should be determined by the user.
Target
- Alternative Names
- Bromodomain Containing 3; RING3-Like Protein; RING3L; Bromodomain-Containing Protein 3 Short Isoform; Bromodomain-Containing Protein 3; Bromodomain-Containing 3; RING3-Like; KIAA0043; ORFX
- Gene ID
- 8019
- UniProt ID
- Q15059
- Long Name
- Bromodomain Containing 3
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-250 | Human Anti-BRD3 Recombinant Antibody (clone D385) | ELISA, IP-MS | Human IgG1 |
| MOB-1485MZ | Recombinant Mouse Anti-Human BRD3 Antibody | IHC-P, WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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