Mouse Anti-Cadherin Recombinant Antibody (clone CH-19)
CAT#: VS-0224-XY18
The recombinant antibody recognizes to pan cadherin and can be used as a marker for the plasma membrane in cells which express cadherins. It can be used for Western blot, Flow cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry-paraffin and Immunohistochemistry-frozen.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/1030_A11_3_selected.jpg
Normal Tissue
Figure 2 Small intestine
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Paneth cells
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_6_2.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_9_3.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (CACO-2, OE19, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079112-CDH17
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Specifications
- Immunogen
- The synthetic peptide, DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponds to amino acids 889-912 of Chicken N-Cadherin (cdh2).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Cadherin
- Species Reactivity
- Human, Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Cow, Cat, Canine, Pig, Xenopus, Snake, Zebrafish
- Clone
- CH-19
- Applications
- Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry-frozen, Immunohistochemistry-paraffin
- Related Disease
- Diffuse Gastric; Lobular Breast Cancer Syndrome
Product Property
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in Western blot (1/1000-1/2000), Flow cytometry (1-2 µl per million cells), Immunofluorescence (1/10-1/200), and Immunohistochemistry (1/10-1/200).
Target
- Alternative Names
- Cadherin; CDH; Calcium-dependent adhesion
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "cadherin"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-146 | Recombinant Anti-cadherin Antibody | WB, Dot, FuncS | IgG |
| MHH-146 | Recombinant Human Anti-cadherin Antibody | ELISA, WB, FC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-146-F(E) | Recombinant Anti-cadherin Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| MHH-146-F(E) | Recombinant Human Anti-cadherin Antibody Fab Fragment | IF, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-146-S(P) | Recombinant Anti-cadherin Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| MHH-146-S(P) | Recombinant Human Anti-cadherin Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
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