Rabbit Anti-CASP6 Recombinant Antibody (VS13-YC129)
CAT#: VS13-YC129
The Rabbit Anti-CASP6 Recombinant Antibody is specific for human, mouse and rat CASP6. CASP6, a downstream enzyme in the caspase activation cascade, undergoes regulation through alternative splicing, involved in apoptosis processing and turning over cellular components. The anti-CASP6 antibody can be used for Western blot (1/1000-1/2000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/400).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11337/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3775/if_selected.jpg
Normal Tissue
Figure 3 Small intestine
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11337/29069_A_5_2_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_7_5.jpg
Normal Tissue
Figure 5 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_4_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24303/53960_A_9_3.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138794-CASP6
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Specifications
- Immunogen
- Recombinant human Apolipoprotein E, aa 210-249.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CASP6
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/400
Target
- Alternative Names
- Caspase 6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; EC 3.4.22.59; MCH2; Apoptotic Protease MCH-2;
- Gene ID
- 839
- UniProt ID
- P55212
- Long Name
- Caspase 6
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_001217.2; NP_116787.1
- Function
- Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:8663580, PubMed:19133298, PubMed:22858542, PubMed:27032039, PubMed:28864531, PubMed:30420425, PubMed:32298652).
Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:8663580, PubMed:9463409, PubMed:11953316, PubMed:17401638).
Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution (PubMed:11953316).
Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis (PubMed:32029622).
Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity).
Involved in intrinsic apoptosis by mediating cleavage of RIPK1 (PubMed:22858542).
Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026).
Cleaves phospholipid scramblase proteins XKR4 and XKR9 (By similarity).
In addition to apoptosis, involved in different forms of programmed cell death (PubMed:32298652).
Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity (PubMed:32298652).
PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis (PubMed:32298652).
Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652).
Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
Regulates B-cell programs both during early development and after antigen stimulation (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CASP6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2086z | Mouse Anti-CASP6 Recombinant Antibody (clone 23H10) | WB, IHC, IP | Mouse IgG1 |
| VS3-CJ1046 | Rabbit Anti-CASP6 Recombinant Antibody (VS3-CJ1046) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY195 | Recombinant Rabbit Anti-CASP6 Antibody (clone R01-3A0) | WB, IHC-P | Rabbit IgG |
| VS3-XY3289 | Rabbit Anti-CASP6 Recombinant Antibody (clone R04-8C2) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS13-YC130 | Rabbit Anti-CASP6 Recombinant Antibody (VS13-YC130) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0477 | Hi-Affi™ Rabbit Anti-CASP6 Recombinant Antibody (clone DS477AB) | FC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY42 | AbPlus™ Anti-CASP6 Magnetic Beads (CBACN-091) | IP, Protein Purification |
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