Mouse Anti-CASP9 Recombinant Antibody (ZG-0365F)
CAT#: ZG-0365F
This product is a mouse antibody that recognizes CASP9.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CASP9 Recombinant Antibody (ZG-0365F) in WB.
Caspase-9 mouse mAb (1:1000 dilution) was used to detect Caspase-9 in A549, MCF7, Jurkat, K562, and Hela cell lysates by Western blot. Predicted band size: 49/37KDa. Observed band size: 49KDa.
WB
Figure 2 Mouse Anti-CASP9 Recombinant Antibody (ZG-0365F) in WB.
Western blot detection of CHO-K1 cell lysate (B) and CHO K1 transfected with Caspase-9 (A) cell lysate was performed using Caspase-9 mouse mAb (1:1000 dilution). Predicted band size: 49/37KDa. Observed band size: 49/37KDa.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1473/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1473/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4348/12208_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4348/12208_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4348/12208_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4348/12208_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4348/12208_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132906-CASP9
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Specifications
- Immunogen
- Recombinant human Caspase-9 protein fragments expressed in
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CASP9
- Species Reactivity
- Human
- Applications
- Western Blot
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Target
- Alternative Names
- Caspase 9; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; ICE-LAP6; APAF-3; MCH6; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; APAF 3; APAF-3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP 9; CASP- 9; CASP9; CASP9_HUMAN; Caspase 9 apoptosis related cysteine peptidase; Caspase 9 Dominant Negative; Caspase 9c; Caspase-9; Caspase-9 subunit p10; Caspase9; ICE LAP6; ICE like apoptotic protease 6; ICELAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9
- Gene ID
- 842
- UniProt ID
- P55211
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| MOB-0422MZ | Mouse Anti-CASP9 Recombinant Antibody (clone 3-33) | IP, WB | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1013 | Anti-CASP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1014 | Anti-Mouse CASP9 Immunohistochemistry Kit | IHC |
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