Anti-CASP9 Products

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For Research Use Only. Not For Clinical Use.

Creative Biolabs is at the forefront of apoptosis and cancer drug discovery, with our elite line of anti-CASP9 recombinant antibodies. As the initiator caspase at the top of the intrinsic, mitochondria-mediated, apoptosis pathway, Caspase-9 (CASP9) is a master regulator of cell suicide. Our premium antibodies provide an essential reagent to monitor mitochondrial dysfunction, the BCL-2 protein family and mechanism of action of many chemotherapeutics.

CASP9: The Apex of the Intrinsic Apoptosis Pathway

As an initiator cysteine protease, Caspase-9 (CASP9) is a central regulator of the intrinsic apoptosis pathway. In response to various cellular insults and DNA damage, pro-apoptotic members of the BCL-2 family trigger the release of the cytochrome c from the mitochondria into the cytosol. In the cytosol, cytochrome c binds to a scaffold protein called Apaf-1, which in turn leads to the assembly of Apaf-1 into a large, wheel-shaped apoptosome complex. The apoptosome then recruits and dimerizes pro-caspase-9, thereby activating it. Activated caspase-9 then cleaves and activates the downstream executioner caspases, mainly caspase-3, committing the cell to die.

Alternative Names

MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Anti-CASP9 rAb Products

Creative Biolabs’ anti-CASP9 recombinant antibodies are the tool of choice for studying the intrinsic apoptosis pathway. We provide you with highly specific antibodies that recognize the pro-form of caspase-9, as well as cleavage-specific antibodies that detect only the large subunit in its activated state. Essential for monitoring activation of the apoptosome by Western Blot, these antibodies provide a definitive readout of mitochondrial death pathway engagement.

Table 1. Featured anti-CASP9 recombinant antibody products at Creative Biolabs.

Creative Quality Control

Creative Biolabs is committed to quality as a company-wide priority. The production of our anti-CASP9 recombinant antibodies is supported by a rigorous quality assurance program. Every lot of our cleavage-specific antibodies is extensively validated for purity, specificity and consistent performance in benchmark models of intrinsic apoptosis so you always receive a reliable reagent.

Customer Reviews

rAb Production

Creative Biolabs employs proprietary production technologies to help power your discoveries in apoptosis. Our anti-cleaved CASP9 recombinant antibodies are carefully developed to only recognize the activated form of the enzyme, for the highest possible specificity and sensitivity for your experiments.

Featured Anti-CASP9 Recombinant Antibody Production Platforms
Fig.3 Milligram-scale anti-CASP9 recombinant antibody production.

Fig.4 Gram-scale anti-CASP9 recombinant antibody production.

rAb Modalities

Creative Biolabs is dedicated to providing innovative and versatile tools for the scientific community. Our anti-CASP9 antibodies are available in a broad selection of modalities to enable a wide range of experimental approaches for studying mitochondrial-driven cell death.

Fig.5 Full-length anti-CASP9 recombinant antibody production and modalities.

Accelerate your apoptosis and cancer drug discovery research with Creative Biolabs’ superior anti-CASP9 recombinant antibodies. Our extensive line of cleavage-specific antibodies is characterized by its unsurpassed specificity and extensive validation, making it the ideal set of tools for your research. Don’t hesitate to let our expertise work for you. Contact one of our specialists today.