Rabbit Anti-CCL27 Recombinant Antibody (clone R66-7R-6) (CAT#: VS3-FY2138)
Clone R66-7R-6 is a rabbit recombinant antibody that detects human CCL27 protein, a calculated weight of 13 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R66-7R-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200), flow cytometry (dilution range 1:50).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 High expression in skin 1
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_9_1_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_9_1_val_selected.jpg
Figure 2 Low expression in skeletal muscle
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_3_6_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_3_6_val_selected.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_A_9_8.jpg
Figure 4 RNA cell line category: Cell line enhanced (AN3-CA)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213927-CCL27
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213927-CCL27
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CCL27
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R66-7R-6
- Applications
- Western Blot, Immunofluorescence, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 13 kDa, while the observed molecular weight stands at 13kDa.
- Related Disease
- Dermatitis and Dermatitis, Atopic
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Flow cytometry: 1/50
Target
- Alternative Names
- C-C Motif Chemokine Ligand 27; CC Chemokine ILC; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 27; Cutaneous T-Cell Attracting Chemokine; Chemokine (C-C Motif) Ligand 27; IL-11 R-Alpha-Locus Chemokine; IL-11 Ralpha-Locus Chemokine; Small-Inducible Cytokine A27; Skinkine; ESKINE
- Gene ID
- 10850
- UniProt ID
- Q9Y4X3
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_006655.1
- Function
- Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CCL27"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2711z | Mouse Anti-CCL27 Recombinant Antibody (clone 12A3) | WB, IHC, Neut | Mouse IgG1 |
| MOB-0359CT | Mouse Anti-CCL27 Recombinant Antibody (clone NN0263-8I55) | IHC-Fr, Neut, WB | Mouse IgG1 |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0091MZ | Chicken Anti-CCL27 Polyclonal IgY | WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-201CQ | Mouse Anti-CCL27 Recombinant Antibody (clone CBL006) | Neut, WB, IHC | Mouse IgG1 |
| NEUT-202CQ | Mouse Anti-CCL27 Recombinant Antibody (clone CBL531) | WB, IHC, Neut | Mouse IgG1 |
| NEUT-203CQ | Mouse Anti-CCL27 Recombinant Antibody (clone CBL532) | WB, Neut | Mouse IgG2b |
| NEUT-204CQ | Mouse Anti-CCL27 Recombinant Antibody (clone MM0152-7H44) | IF, WB, IHC-Fr, Neut | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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