Rabbit Anti-CCL27 Recombinant Antibody (clone R66-7R-6) (CAT#: VS3-FY2138)
Clone R66-7R-6 is a rabbit recombinant antibody that detects human CCL27 protein, a calculated weight of 13 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R66-7R-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200), flow cytometry (dilution range 1:50).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 High expression in skin 1
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_9_1_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_9_1_val_selected.jpg
Figure 2 Low expression in skeletal muscle
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_3_6_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_B_3_6_val_selected.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73848/165690_A_9_8.jpg
Figure 4 RNA cell line category: Cell line enhanced (AN3-CA)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213927-CCL27
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213927-CCL27
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CCL27
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R66-7R-6
- Applications
- Western Blot, Immunofluorescence, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 13 kDa, while the observed molecular weight stands at 13kDa.
- Related Disease
- Dermatitis and Dermatitis, Atopic
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Flow cytometry: 1/50
Target
- Alternative Names
- C-C Motif Chemokine Ligand 27; CC Chemokine ILC; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 27; Cutaneous T-Cell Attracting Chemokine; Chemokine (C-C Motif) Ligand 27; IL-11 R-Alpha-Locus Chemokine; IL-11 Ralpha-Locus Chemokine; Small-Inducible Cytokine A27; Skinkine; ESKINE
- Gene ID
- 10850
- UniProt ID
- Q9Y4X3
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_006655.1
- Function
- Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2711z | Mouse Anti-CCL27 Recombinant Antibody (clone 12A3) | WB, IHC, Neut | Mouse IgG1 |
| MOB-0359CT | Mouse Anti-CCL27 Recombinant Antibody (clone NN0263-8I55) | IHC-Fr, Neut, WB | Mouse IgG1 |
| VS7-HM310 | Mouse Anti-CCL27 Recombinant Antibody (clone 3B7A5) | IHC, ICC, FCM | Mouse IgG1 |
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