Rabbit Anti-CCNH Recombinant Antibody (clone R02-4I4) (CAT#: VS3-FY1993)
This is a rabbit recombinant monoclonal antibody, which binds to human CCNH. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This R02-4I4 antibody is validated for western blot analysis of Cyclin H/p34 in 3T3 lysates and western blot analysis of Cyclin H/p34 in U251, HL-60, Lncap lysates using cyclin H/p34 antibody.
Specific Inquiry
WB
Figure 1 Anti-CCNH Antibody (VS3-FY1993) in WB.
The Cyclin H/p34 expression in 3T3 lysates were detected using the anti-Cyclin H/p34 by western blot assay.
WB
Figure 2 Anti-CCNH Antibody (VS3-FY1993) in WB.
The Cyclin H/p34 expression in U251, HL-60, Lncap lysates were detected using the anti-Cyclin H/p34 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human Cyclin H/p34
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CCNH
- Species Reactivity
- Human, Mouse
- Clone
- R02-4I4
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 38 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin H; MO15-Associated Protein; Cyclin-Dependent Kinase-Activating Kinase Complex Subunit; CDK-Activating Kinase Complex Subunit; CAK Complex Subunit; P34
- Gene ID
- 902
- UniProt ID
- P51946
- Sequence Similarities
- Belongs to the cyclin family. Cyclin C subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys72 (NX_P51946 [NX_P51946-1])
Modification sites at PhosphoSitePlus (P51946)
Glycosylation from GlyGen (P51946) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047273819.1; XP_054209722.1; NP_001186118.1; NP_001230.1; NP_001350468.1
- Function
- Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0115CT | Mouse Anti-CCNH Recombinant Antibody (clone EML256) | IF, IHC-P, WB | Mouse IgG2a |
| MRO-0418-CN | Rabbit Anti-CCNH Recombinant Antibody (clone CBACN-168) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-QX176 | Mouse Anti-CCNH Recombinant Antibody (clone 1A7-B5-D4) | WB | Mouse IgG2b |
| VS3-CJ1027 | Rabbit Anti-CCNH Recombinant Antibody (VS3-CJ1027) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY2651 | Mouse Anti-CCNH Recombinant Antibody (VS3-FY2651) | WB, IP | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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