Rabbit Anti-CCNH Recombinant Antibody (clone R02-4I4)
CAT#: VS3-FY1993
This is a rabbit recombinant monoclonal antibody, which binds to human CCNH. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This R02-4I4 antibody is validated for western blot analysis of Cyclin H/p34 in 3T3 lysates and western blot analysis of Cyclin H/p34 in U251, HL-60, Lncap lysates using cyclin H/p34 antibody.
Tested Data
Gene Expression
WB
Figure 1 Anti-CCNH Antibody (VS3-FY1993) in WB.
The Cyclin H/p34 expression in 3T3 lysates were detected using the anti-Cyclin H/p34 by western blot assay.
WB
Figure 2 Anti-CCNH Antibody (VS3-FY1993) in WB.
The Cyclin H/p34 expression in U251, HL-60, Lncap lysates were detected using the anti-Cyclin H/p34 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44138/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19416/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19416/42340_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19416/42340_A_8_5.jpg
Normal Tissue
Figure 5 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19416/42340_A_4_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19416/42340_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134480-CCNH
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Specifications
- Immunogen
- Synthetic peptide of human Cyclin H/p34
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CCNH
- Species Reactivity
- Human, Mouse
- Clone
- R02-4I4
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 38 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin H; MO15-Associated Protein; Cyclin-Dependent Kinase-Activating Kinase Complex Subunit; CDK-Activating Kinase Complex Subunit; CAK Complex Subunit; P34
- Gene ID
- 902
- UniProt ID
- P51946
- Sequence Similarities
- Belongs to the cyclin family. Cyclin C subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys72 (NX_P51946 [NX_P51946-1])
Modification sites at PhosphoSitePlus (P51946)
Glycosylation from GlyGen (P51946) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047273819.1; XP_054209722.1; NP_001186118.1; NP_001230.1; NP_001350468.1
- Function
- Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CCNH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0115CT | Mouse Anti-CCNH Recombinant Antibody (clone EML256) | IF, IHC-P, WB | Mouse IgG2a |
| ZG-0449F | Mouse Anti-CCNH Recombinant Antibody (ZG-0449F) | WB | Mouse IgG |
| ZG-0450F | Mouse Anti-CCNH Recombinant Antibody (ZG-0450F) | WB | Mouse IgG |
| VS3-CJ1027 | Rabbit Anti-CCNH Recombinant Antibody (VS3-CJ1027) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS4-WK71 | Mouse Anti-CCNH Recombinant Antibody (VS4-WK71) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0519 | Hi-Affi™ Rabbit Anti-CCNH Recombinant Antibody (clone DS519AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1092 | Anti-CCNH Immunohistochemistry Kit | IHC | |
| VS-0525-XY1093 | Anti-Mouse CCNH Immunohistochemistry Kit | IHC |
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