Mouse Anti-CD163 Recombinant Antibody (VS3-FY2652)
CAT#: VS3-FY2652
The anti-CD163 antibody is a mouse recombinant monoclonal antibody, which has been intricately crafted to target the CD163 protein, a calculated weight of 125.4 kDa. It shows capability of cross-reacting with human and mouse. This monoclonal antibody is potential for conducting research on Rosai-Dorfman Disease and Non-Langerhans-Cell Histiocytosis. This monoclonal antibody can be used in Western Blot, Immunohistochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/1373_A6_3_selected.jpg
Normal Tissue
Figure 2 Lung
Macrophages
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/134927_A_3_4.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/134927_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46404/134927_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (GAMG, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177575-CD163
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- CD163 protein of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 125.4 kDa.
- Related Disease
- Rosai-Dorfman Disease and Non-Langerhans-Cell Histiocytosis
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- CD163 Molecule; Hemoglobin Scavenger Receptor; CD163 Antigen; M130; Scavenger Receptor Cysteine-Rich Type 1 Protein M130; Macrophage-Associated Antigen; SCARI1; MM130
- Gene ID
- 9332
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma.
Phosphorylated.
Glycosylation at Asn105, Asn123, Asn140, Asn445, Asn767, Asn1001, and Asn1027
Modification sites at PhosphoSitePlus
Glycosylation from Gly
Glycosylation from GlyGen 11 sites, 14 N-linked glycans (6 sites), 2 O-linked glycans (3 sites)
- Protein Refseq
- XP_024305046.1; XP_047285851.1; XP_054229836.1; XP_054229837.1; NP_001357074.1
- Function
- May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme.
Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner.
Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CD163"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-164-F(E) | Recombinant Anti-human CD163 Antibody Fab Fragment | RIA, IP, FuncS | Fab |
| MHH-164-F(E) | Recombinant Human Anti-human CD163 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-164-S(P) | Recombinant Anti-human CD163 Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
| MHH-164-S(P) | Recombinant Human Anti-human CD163 Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0535 | Hi-Affi™ Rabbit Anti-CD163 Recombinant Antibody (clone DS535AB) | IHC-P, IHC-Fr | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0282-CN | Rabbit Anti-CD163 Recombinant Antibody (clone JA51-30) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-WK1051 | Mouse Anti-CD163 Recombinant Antibody (VS3-WK1051) | IHC | Mouse IgG1 |
| VS3-WK1052 | Mouse Anti-CD163 Recombinant Antibody (clone ABT-CD163) | IHC-P | Mouse IgG1, κ |
| VS4-WK77 | Mouse Anti-CD163 Recombinant Antibody (VS4-WK77) | ELISA, IHC | Mouse IgG1, Kappa |
| MOB-388YC | Rat Anti-CD163 Recombinant Antibody (MOB-388YC) | ELISA, FC | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC520 | AbPlus™ Anti-CD163 Magnetic Beads (VS-0724-YC520) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY81 | Mouse Anti-NHP CD163 Recombinant Antibody (clone EDHu-1) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY354 | Anti-CD163 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1120 | Anti-Human CD163 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1121 | Anti-Rat CD163 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC153 | CytoStream™ Rabbit Anti-CD163 Polyclonal Antibody (VS13-YC153) | WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF | Rabbit IgG |
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