AbPlus™ Anti-CD58 Magnetic Beads (VS-0724-YC604)
CAT#: VS-0724-YC604
The AbPlus Anti-CD58 Magnetic Beads (VS-0724-YC604) is an innovative affinity resin which is bound with anti-CD58 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of CD58 protein under native and denaturing conditions.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116815-CD58
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-CD58 specific antibody
- Target
- CD58
- Immunogen
- Recombinant human LFA-3/CD58, aa 1-215.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- CD58 (CD58 Molecule) is a Protein Coding gene. Diseases associated with CD58 include Whipple Disease and Oral Lichen Planus. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System. Gene Ontology (GO) annotations related to this gene include receptor binding.
- Alternative Names
- CD58 Molecule; CD58 Antigen, (Lymphocyte Function-Associated Antigen 3); Surface Glycoprotein LFA-3; LFA3; AG3; Lymphocyte Function-Associated Antigen 3; CD58 Antigen; LFA-3;
- Gene ID
- 965
- UniProt ID
- P19256
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High Yield Protein Isolation
We needed to isolate CD58 from cell lysates to study its interaction with CD2. These beads provided a high yield of the target protein, and the elution process was gentle enough to preserve the protein-protein interactions for our subsequent analysis.
Convenient Magnetic Separation
The magnetic separation format is much faster than traditional column methods. It saved us a lot of hands-on time during the sample preparation step, and the beads responded very quickly to the magnetic stand we have in the lab.
Q&As
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What is the primary target of these magnetic beads?
A: These beads are conjugated with an antibody specific for CD58, also known as Lymphocyte Function-associated Antigen 3 (LFA-3). CD58 is an adhesion molecule involved in immune cell interactions, particularly binding to CD2, making these beads useful for immunology research.
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How does the magnetic bead format benefit protein isolation?
A: The magnetic bead format allows for rapid and gentle isolation of the target protein. Compared to traditional column chromatography or centrifugation methods, magnetic separation is faster and reduces mechanical stress on the sample, helping to preserve the native conformation and interactions of the CD58 protein.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| MOB-1415z | Mouse Anti-CD58 Recombinant Antibody (clone 40C4) | WB, IP, FC | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-1837-FY-F(E) | Mouse Anti-CD58 Recombinant Antibody (clone 1E6); scFv Fragment | FC, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
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| HPAB-1837-FY-S(P) | Mouse Anti-CD58 Recombinant Antibody (clone 1E6); Fab Fragment | FC, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS2-QX19 | Mouse Anti-CD58 Recombinant Antibody (clone BRIC 5) | IB | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC170 | CytoStream™ Rabbit Anti-CD58 Recombinant Antibody (VS13-YC170) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC507 | Recombinant Anti-CD58 Vesicular Antibody, EV Displayed (VS-0425-YC507) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1202 | Anti-CD58 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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