Rabbit Anti-CD81 Recombinant Antibody (clone 9F7)
CAT#: ZG-0596J
This product is a rabbit antibody that recognizes CD81.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-CD81 Antibody (ZG-0596J) in WB
Western Blot
Positive WB detected in:K562 whole cell lysate
All lanes:CD81 antibody at 1.25μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 KDa
Observed band size: 26 KDa
IHC
Figure 2 Rabbit Anti-CD81 Antibody (ZG-0596J) in IHC
IHC image of ZG-0596J diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 3 Rabbit Anti-CD81 Antibody (ZG-0596J) in IHC
IHC image of ZG-0596J diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
FC
Figure 4 Rabbit Anti-CD81 Antibody (ZG-0596J) in FC
Overlay histogram showing Jurkat cells stained with ZG-0596J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7234/10_B9_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_A_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_A_5_6.jpg
Normal Tissue
Figure 5 Endometrium
Cells in endometrial stroma
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2507/6520_B_3_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110651-CD81
❮
❯
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD81
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 9F7
- Applications
- ELISA, WB, IHC, FC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.
WB:1:500-1:5000, IHC:1:50-1:500
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0596J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CD81"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-036 | Mouse Anti-CD81 Recombinant Antibody (clone K04) | WB, Block, FuncS | Mouse IgG |
| MOB-1539MZ | Recombinant Mouse Anti-Human CD81 Antibody (clone N49), PE-Conjugated | FC, FuncS, ICC, IHC-P, IP, WB | Mouse antibody |
| HPAB-0908-YJ | Mouse Anti-CD81 Recombinant Antibody (HPAB-0908-YJ) | ELISA, FC, Inhib | Mouse IgG1 |
| FAMAB-1086CQ | Human Anti-CD81 Recombinant Antibody (clone 1D6) | IB, FC | Human IgG1 |
| FAMAB-1087CQ | Human Anti-CD81 Recombinant Antibody (clone CD81K04) | Neut, FC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-036 | Mouse Anti-CD81 Recombinant Antibody (clone K04); scFv Fragment | WB, Block, FuncS | Mouse scFv |
| PSBL-425 | Mouse Anti-CD81 Recombinant Antibody (clone K13); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| HPAB-0139-FY-S(P) | Human Anti-CD81 Recombinant Antibody (clone 002-A07 S115N); scFv Fragment | Inhib | Human scFv |
| PSBC-257 | Mouse Anti-CD81 Recombinant Antibody (clone Ab4); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-0908-YJ-S(P) | Mouse Anti-CD81 Recombinant Antibody; scFv Fragment (HPAB-0908-YJ-S(P)) | ELISA, FC, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-036 | Mouse Anti-CD81 Recombinant Antibody (clone K04); Fab Fragment | WB, Block, FuncS | Mouse Fab |
| PFBC-257 | Mouse Anti-CD81 Recombinant Antibody (clone Ab4); Fab Fragment | ELISA, FC | Mouse Fab |
| PFBC-258 | Mouse Anti-CD81 Recombinant Antibody (clone Ab5); Fab Fragment | ELISA, FC | Mouse Fab |
| PFBC-259 | Mouse Anti-CD81 Recombinant Antibody (clone Ab10); Fab Fragment | ELISA, FC | Mouse Fab |
| MOB-164YJL-F(E) | Human Anti-CD81 Recombinant Antibody (clone CBC083); Bivalent Fab Fragment | ELISA, FC | Human Bivalent Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-348LC | Human Anti-CD81 Recombinant Antibody (TAB-348LC) | ELISA | Human IgG4 |
| TAB-348LC-S(P) | Human Anti-CD81 Recombinant Antibody; scFv Fragment (TAB-348LC-S(P)) | ELISA | Human scFv |
| TAB-349LC-S(P) | Human Anti-CD81 Recombinant Antibody; scFv Fragment (TAB-349LC-S(P)) | ELISA | Human scFv |
| TAB-348LC-F(E) | Human Anti-CD81 Recombinant Antibody; Fab Fragment (TAB-348LC-F(E)) | ELISA | Human Fab |
| TAB-349LC-F(E) | Human Anti-CD81 Recombinant Antibody; Fab Fragment (TAB-349LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-417CQ | Hamster Anti-CD81 Recombinant Antibody (clone 2F7) | Neut, FC, IHC, IHC-Fr, IP, ICC | Hamster IgG3, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-418CQ | Hamster Anti-Cd81 Recombinant Antibody (clone YC081), Biotin | FC, IHC-Fr, IP, Block | Hamster IgG3, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0584 | Hi-Affi™ Rabbit Anti-CD81 Recombinant Antibody (clone DS584AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY74 | CytoStream™ Mouse Anti-CD81 Recombinant Antibody (VS-0225-XY74) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC426 | Recombinant Anti-CD81 Vesicular Antibody, EV Displayed (VS-0425-YC426) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1228 | Anti-CD81 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1229 | Anti-Mouse CD81 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1230 | Anti-Rat CD81 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, SPR, Inhib, FuncS
-3.jpg)
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, IHC, Activ, FuncS, IF, ELISA
-2.png)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
