Rabbit Anti-CDC6 Recombinant Antibody (clone R03-5K5)
CAT#: VS3-XY3303
The recombinant rabbit antibody is specific for CDC6 (Cell division cycle 6), which involves in the initiation of DNA replication. CDC6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. The antibody specifically reacts with human CDC6. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm, cytosol, cytokinetic bridge & mitotic spindle.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65070/1715_F3_5_cr57fd1233a1185_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach, upper
Immunohistochemical staining of human stomach, upper shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_A_4_6.jpg
Normal Tissue
Figure 8 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_B_2_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50114/116989_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000094804-CDC6
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDC6
- Species Reactivity
- Human
- Clone
- R03-5K5
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Meier-Gorlin syndrome 5 (MGORS5)
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- CDC18L; HsCDC6; MGORS5; HsCDC18
- Gene ID
- 990
- UniProt ID
- Q99741
- Long Name
- Cell division cycle 6
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex.
- Protein Refseq
- XP_011523844.1; XP_011523843.1
- Function
- Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDC6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0517CT | Recombinant Mouse anti-Human CDC6 Monoclonal antibody (EDT-290) | IP, WB | |
| VS3-WK1120 | Rabbit Anti-CDC6 Recombinant Antibody (VS3-WK1120) | WB, ICC, IF | Rabbit IgG |
| VS3-WK1998 | Rabbit Anti-CDC6 (pS106) Recombinant Antibody (clone R01-0F2) | WB | Rabbit IgG |
| VS3-WK2157 | Rabbit Anti-CDC6 (pS54) Recombinant Antibody (clone R04-6V2) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0607 | Hi-Affi™ Rabbit Anti-CDC6 Recombinant Antibody (clone DS607AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1270 | Anti-CDC6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1271 | Anti-Yeast CDC6 Immunohistochemistry Kit | IHC |
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