Rabbit Anti-CDK2 (pY15) Recombinant Antibody (clone R05-2C4)
CAT#: VS3-WK1981
This product is a rabbit recombinant antibody that is specific to phosphorylated CDK2. The target of this antibody is phospho-CDK2 (Tyr15). The clone R05-2C4 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of CDK2 (pTyr15) observed in WB experiments using this antibody is 34 kDa.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_6_6.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_8_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Low Intensity: Moderate Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123374-CDK2
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13115/32765_A_7_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123374-CDK2
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WB
Figure 1 Rabbit Anti-CDK2 (pTyr15) Recombinant Antibody (clone R05-2C4) in WB.
Western blot analysis of phospho-CDK2 (Tyr15) in Jurkat, CHO-K1, Hela lysates using anti-CDK2 (pTyr15) antibody, Cat# VS3-WK1981.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Tyr15 of human Cdk2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr15 of human, hamster and rat CDK2
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R05-2C4
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
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Related Signaling Pathways
p53 Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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