Chicken Anti-CENPB Recombinant Antibody (clone 5D1); scFv Fragment
CAT#: FAMAB-0002JF-S(P)
Recombinant chicken monoclonal antibody specially binds to human CENP-B. This anti-CENP-B antibody 5D1 can be potentially wildly used in the treatment of systemic sclerosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53507/1874_F11_35_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/54405/877_A3_1_selected.jpg
Subcellular Location
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11626/27766_A_6_5_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53507/819_E10_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11626/27721_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11626/27766_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11626/27766_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11626/27766_A_7_5.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11626/27766_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000125817-CENPB
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Specifications
- Immunogen
- Antigen mixture 1 (annexin XI, centromere protein B, DNA topoisomerase I, histidyl tRNA synthetase, Ro60, Rpp38 and U1A) and mixture 2 (a-fodrin, human Pop1 protein, heat shock protein B3, Ro52, Rpp30, Rrp4 and Rrp42)
- Host Species
- Chicken
- Derivation
- Chicken phage display scFv library
- Type
- Chicken scFv
- Specificity
- Human CENPB
- Species Reactivity
- Human
- Clone
- 5D1
- Applications
- IB, WB, ELISA, FuncS
- Related Disease
- Systemic sclerosis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 5D1"
- CAT
- Product Name
See other products for "CENPB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1127z | Mouse Anti-CENPB Recombinant Antibody (clone 25B8) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| FAMAB-0002JF | Chicken Anti-CENPB Recombinant Antibody (clone 5D1) | WB, ELISA, Inhib, IHC, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0662 | Hi-Affi™ Rabbit Anti-CENPB Recombinant Antibody (clone DS662AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0002JF-F(E) | Chicken Anti-CENPB Recombinant Antibody (clone 5D1); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
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