Chicken Anti-CENPB Recombinant Antibody (clone 5D1) (CAT#: FAMAB-0002JF)
Recombinant chicken monoclonal antibody specially binds to human CENP-B. This anti-CENP-B antibody 5D1 can be potentially wildly used in the treatment of systemic sclerosis.
Specific Inquiry
WB
Figure 1 Human epithelial cell line (HeLa) extracts were prepared by sonication and Western blotted onto nitrocellulose membrane.
Molecular weights (kDa) of marker proteins are indicated on the left.
Hof, D., Hoeke, M. O., & Raats, J. M. H. (2008). Multiple‐antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens. Clinical & Experimental Immunology, 151(2), 367-377.
ELISA
Figure 2 Chicken serum enzyme-linked immunosorbent assay (ELISA).
Pre-immune and immune sera of chickens 1 and 2 were tested for reactivity with centromere protein B.
Hof, D., Hoeke, M. O., & Raats, J. M. H. (2008). Multiple‐antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens. Clinical & Experimental Immunology, 151(2), 367-377.
IHC
Figure 3 Immunostaining of human colon tissue with anti CENP-B antibodies.
At least 300 dots of cells were counted.
Tomonaga, T., Matsushita, K., Yamaguchi, S., Oohashi, T., Shimada, H., Ochiai, T., ... & Nomura, F. (2003). Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer. Cancer research, 63(13), 3511-3516.
IF
Figure 4 CENP-B is degraded in the TR-ICP0.39 cell line.
Immunofluorescence study showing the correct ICP0 expression and activity in the TRICP0.39 (TR-ICP0) cell line.
Tomonaga, T., Matsushita, K., Yamaguchi, S., Oohashi, T., Shimada, H., Ochiai, T., ... & Nomura, F. (2003). Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer. Cancer research, 63(13), 3511-3516.
Inhib
Figure 5 ICP0 induces the degradation of cenpB in mouse cells.
Anti-CENP-B was used to specifically detect cenpB (green).
Tomonaga, T., Matsushita, K., Yamaguchi, S., Oohashi, T., Shimada, H., Ochiai, T., ... & Nomura, F. (2003). Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer. Cancer research, 63(13), 3511-3516.
Specifications
- Immunogen
- Antigen mixture 1 (annexin XI, centromere protein B, DNA topoisomerase I, histidyl tRNA synthetase, Ro60, Rpp38 and U1A) and mixture 2 (a-fodrin, human Pop1 protein, heat shock protein B3, Ro52, Rpp30, Rrp4 and Rrp42)
- Host Species
- Chicken
- Derivation
- Chicken phage display scFv library
- Specificity
- Human CENPB
- Species Reactivity
- Human
- Clone
- 5D1
- Applications
- WB, ELISA, Inhib, IHC, IF
- Related Disease
- Systemic sclerosis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CENPB antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Inhibition, Immunofluorescence.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 5D1"
See other products for "CENPB"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1127z | Mouse Anti-CENPB Recombinant Antibody (clone 25B8) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0662 | Hi-Affi™ Rabbit Anti-CENPB Recombinant Antibody (clone DS662AB) | WB, IP | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0002JF-F(E) | Chicken Anti-CENPB Recombinant Antibody (clone 5D1); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0002JF-S(P) | Chicken Anti-CENPB Recombinant Antibody (clone 5D1); scFv Fragment | IB, WB, ELISA, FuncS | Chicken scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for FAMAB-0002JF. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.