Rabbit Anti-CENPE Recombinant Antibody (clone R06-1G4) (CAT#: VS3-FY1682)
This product is purified by affinity chromatography. It is a extraordinary rabbit recombinant monoclonal antibody that is used to detect human CENPE. The calculated molecular weight of the protein is 316 kDa, while the observed molecular weight of the protein is also 316 kDa. The R06-1G4 is recommended for dilution with western blot (dilution 1/500-1/1000).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_9_4.jpg
Figure 2 Kidney
(Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_7_5.jpg
(Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_7_5.jpg
Figure 3 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_6_6.jpg
Figure 4 Tonsil
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_4_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42294/167979_A_4_8.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138778-CENPE
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138778-CENPE
Specifications
- Immunogen
- Synthetic peptide of human CENPE
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CENPE
- Species Reactivity
- Human
- Clone
- R06-1G4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 316 kDa, while the observed molecular weight stands at 316 kDa.
- Related Disease
- Microcephaly 13, Primary, Autosomal Recessive and Seckel Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Centromere Protein E; Protein Phosphatase 1, Regulatory Subunit 61; Kinesin-Related Protein CENPE; Centromere Protein E, 312kDa; Kinesin-7; CENP-E; Centromere Autoantigen E (312kD)
- Gene ID
- 1062
- UniProt ID
- Q02224
- Sequence Similarities
- Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
- Cellular Localization
- Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule
- Post Translation Modifications
- The C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor ().
Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore.
- Protein Refseq
- XP_011529846.1; XP_011529847.1; XP_011529848.1; XP_011529849.1; XP_011529850.1
- Function
- Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CENPE"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2503z | Mouse Anti-CENPE Recombinant Antibody (clone 6C1) | WB, ELISA, ICC, IF, IP | Mouse IgG1 |
| MOB-2481MZ | Recombinant Mouse Anti-Human CENPE Antibody (clone 2I23) | FC, ICC/IF, IP, WB | Mouse antibody |
| VS1-YJ32 | Mouse Anti-CENPE Recombinant Antibody (clone 1H12) | ICC, IF, IP, ICC, FC | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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