Mouse Anti-CHD3 Recombinant Antibody (VS3-CJ76) (CAT#: VS3-CJ76)
This product is a mouse antibody that recognizes CHD3.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
Figure 3 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
Figure 4 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
Figure 6 Colon
(Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
(Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
Figure 7 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
Specifications
- Immunogen
- Recombinant fragment of human CHD3 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CHD3
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH
- Gene ID
- 1107
- UniProt ID
- Q12873
- Sequence Similarities
- Belongs to the SNF2/RAD54 helicase family.
- Cellular Localization
- Nucleus, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression.
- Protein Refseq
- NP_001005271.2; NP_001005273.1; NP_005843.2
- Function
- Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones (PubMed:9804427, PubMed:30397230).
Involved in transcriptional repressiobn as part of the NuRD complex (PubMed:27068747).
Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity (PubMed:17626165).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CHD3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0486CT | Recombinant Mouse anti-Human CHD3 Monoclonal antibody (EML044) | IHC-P, WB | |
| ZG-0416F | Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) | WB, ICC | Mouse IgG |
| VS3-QX232 | Mouse Anti-CHD3 Recombinant Antibody (clone 4B1-F1-G4) | WB, IHC, FC | Mouse IgG1 |
| VS3-QX233 | Mouse Anti-CHD3 Recombinant Antibody (clone 7F6-G2-E5) | WB, ICC, IP | Mouse IgG1 |
| VS3-XY497 | Mouse Anti-CHD3 Recombinant Antibody (clone 5B2E5) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0685 | Hi-Affi™ Rabbit Anti-CHD3 Recombinant Antibody (clone DS685AB) | WB, IHC, ICC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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