Mouse Anti-CHD3 Recombinant Antibody (VS3-CJ76)
CAT#: VS3-CJ76
This product is a mouse antibody that recognizes CHD3.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
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Specifications
- Immunogen
- Recombinant fragment of human CHD3 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CHD3
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- Mi-2a; Mi2-ALPHA; SNIBCPS; ZFH
- Gene ID
- 1107
- UniProt ID
- Q12873
- Sequence Similarities
- Belongs to the SNF2/RAD54 helicase family.
- Cellular Localization
- Nucleus, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression.
- Protein Refseq
- NP_001005271.2; NP_001005273.1; NP_005843.2
- Function
- Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones (PubMed:9804427, PubMed:30397230).
Involved in transcriptional repressiobn as part of the NuRD complex (PubMed:27068747).
Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity (PubMed:17626165).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "CHD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-299 | Human Anti-CHD3 Recombinant Antibody (clone D404) | ELISA, IP-MS | Human IgG |
| MOB-0486CT | Recombinant Mouse anti-Human CHD3 Monoclonal antibody (EML044) | IHC-P, WB | |
| ZG-0416F | Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) | WB, ICC | Mouse IgG |
| VS3-XY497 | Mouse Anti-CHD3 Recombinant Antibody (clone 5B2E5) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-XY3313 | Rabbit Anti-CHD3 Recombinant Antibody (clone R05-6G3) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0685 | Hi-Affi™ Rabbit Anti-CHD3 Recombinant Antibody (clone DS685AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY443 | Anti-CHD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1425 | Anti-Mouse CHD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1424 | Anti-Human CHD3 Immunohistochemistry Kit | IHC |
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