Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F)
CAT#: ZG-0416F
This product is a mouse antibody that recognizes the epitope C-terminus of the CHD3.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) in WB.
Western blot detection of CHD3 (C-terminal) in NTERA2, 3T3, C6, K562 and Hela cell lysates using CHD3 (C-terminal) mouse mAb (1:1000 dilution). Predicted band size: 260KDa. Observed band size: 260KDa.
ICC
Figure 2 Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) in ICC.
Immunocytochemical staining of HeLa cells using -20™ methanol and CHD3 (C-terminal) mouse mAb (diluted 1:400).
IP
Figure 3 Mouse Anti-CHD3 Recombinant Antibody (ZG-0416F) in IP.
Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Fibroblasts
Staining:High
Intensity: Strong
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3
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Specifications
- Immunogen
- Recombinant human CHD3 (C-terminus) protein fragments
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human CHD3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunocytochemistry
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Immunocytochemistry: 1:400
Target
- Alternative Names
- Chromodomain Helicase DNA Binding Protein 3; Mi-2 Autoantigen 240 KDa Protein; ATP-Dependent Helicase CHD3; Zinc Finger Helicase; Mi2-ALPHA; CHD-3; HZFH; 226; 2600010P09Rik; AF020312; ATP dependent helicase CHD3; ATP_x0002_dependent helicase Chd3; CHD- 3; Chd3; CHD3_HUMAN; Chd7; Chromodomain-helicase-DNA-binding protein 3; hZFH; MGC40857; Mi 2 autoantigen 240 kDa protein; Mi 2a; Mi-2 autoantigen 240 kDa protein; Mi2 ALPHA; Mi2-alpha; Prp7; Prp9 1; ZFH; Zinc finger helicase; zinc-finger helicase (Snf2-like).
- Gene ID
- 1107
- UniProt ID
- Q12873
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| DrMAB-299 | Human Anti-CHD3 Recombinant Antibody (clone D404) | ELISA, IP-MS | Human IgG |
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| MOB-0486CT | Recombinant Mouse anti-Human CHD3 Monoclonal antibody (EML044) | IHC-P, WB |
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| MOR-0685 | Hi-Affi™ Rabbit Anti-CHD3 Recombinant Antibody (clone DS685AB) | WB, IHC, ICC | Rabbit IgG |
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| VS3-QX232 | Mouse Anti-CHD3 Recombinant Antibody (clone 4B1-F1-G4) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS3-QX233 | Mouse Anti-CHD3 Recombinant Antibody (clone 7F6-G2-E5) | WB, ICC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS3-CJ76 | Mouse Anti-CHD3 Recombinant Antibody (VS3-CJ76) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY443 | Anti-CHD3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY1425 | Anti-Mouse CHD3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY1424 | Anti-Human CHD3 Immunohistochemistry Kit | IHC |
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