Rabbit Anti-CHEK1 (pS280) Recombinant Antibody (clone R32-4D-1)
CAT#: VS3-WK2092
This product is a rabbit recombinant antibody that is specific to phosphorylated CHEK1. The target of this antibody is phospho-CHEK1 (Serer280). The clone R32-4D-1 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of CHEK1 (pSerer280) observed in WB experiments using this antibody is 54 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149554-CHEK1
Subcellular Location
Figure 2 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/804_D1_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/712_D1_1_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149554-CHEK1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Serer280 of human CHEK1
- Species Reactivity
- Human
- Clone
- R32-4D-1
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "CHEK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0688 | Hi-Affi™ Rabbit Anti-CHEK1 Recombinant Antibody (clone DS688AB) | IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0419F | Mouse Anti-CHEK1 Recombinant Antibody (ZG-0419F) | WB, ELISA | Mouse IgG |
| VS3-CJ127 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ127) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-CJ128 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ128) | WB, IHC | Rabbit IgG |
| VS3-XY498 | Mouse Anti-CHEK1 Recombinant Antibody (clone 2G1D5) | ELISA, WB | Mouse IgG1 |
| VS3-FY376 | Recombinant Rabbit Anti-CHEK1 Antibody (clone R07-4K2) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY120 | Mouse Anti-NHP CHEK1 Recombinant Antibody (clone T.59.2) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY38 | Mouse Anti-CHEK1 scFv-Fc Chimera (VS-0125-FY38) | FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC216 | CytoStream™ Rabbit Anti-CHEK1 Recombinant Antibody (VS13-YC216) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1428 | Anti-CHEK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1429 | Anti-Mouse CHEK1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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