CytoStream™ Rabbit Anti-CHEK1 Recombinant Antibody (VS13-YC216)
CAT#: VS13-YC216
The CytoStream™ Rabbit Anti-CHEK1 Recombinant Antibody is specific for human and mouse CHEK1. CHEK1 aids in cell cycle checkpoint regulation responding to DNA damage, interacting with various proteins to mitigate genomic instability. The anti-CHEK1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149554-CHEK1
Subcellular Location
Figure 2 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/804_D1_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/712_D1_1_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149554-CHEK1
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Specifications
- Immunogen
- Recombinant human Chk1, aa 431-476.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CHEK1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Checkpoint Kinase 1; Cell Cycle Checkpoint Kinase; CHK1 Checkpoint Homolog; EC 2.7.11.1; CHK1; Serine/Threonine-Protein Kinase Chk1; Checkpoint, S. Pombe, Homolog Of, 1;
- Gene ID
- 1111
- UniProt ID
- O14757
- Long Name
- Checkpoint Kinase 1
- Cellular Localization
- Chromosome; Cytoplasm; Cytoskeleton; Nucleus
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CHEK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0688 | Hi-Affi™ Rabbit Anti-CHEK1 Recombinant Antibody (clone DS688AB) | IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0359-CN | Rabbit Anti-CHEK1 Recombinant Antibody (clone CBACN-146) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0419F | Mouse Anti-CHEK1 Recombinant Antibody (ZG-0419F) | WB, ELISA | Mouse IgG |
| VS3-CJ127 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ127) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-CJ128 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ128) | WB, IHC | Rabbit IgG |
| VS3-XY3314 | Rabbit Anti-CHEK1 Recombinant Antibody (clone R02-1D6) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY120 | Mouse Anti-NHP CHEK1 Recombinant Antibody (clone T.59.2) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY38 | Mouse Anti-CHEK1 scFv-Fc Chimera (VS-0125-FY38) | FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1428 | Anti-CHEK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1429 | Anti-Mouse CHEK1 Immunohistochemistry Kit | IHC |
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