Rabbit Anti-CHGA Recombinant Antibody (clone R01-7E1)
CAT#: VS3-FY1685
This product is a extraordinary rabbit recombinant monoclonal antibody that is used to detect human and rat CHGA. This antibody is suitable for validation in immunohistochemistry (IHC) studies. The R01-7E1 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17369/1734_B3_2_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23/250_A_8_3.jpg
Normal Tissue
Figure 3 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17369/141310_A_3_3.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100604-CHGA
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Specifications
- Immunogen
- Synthetic peptide of human chromogranin A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CHGA
- Species Reactivity
- Human, Rat
- Clone
- R01-7E1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 51 kDa, while the observed molecular weight stands at 92 kDa.
- Related Disease
- Acinar Cell Carcinoma and Neuroendocrine Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Chromogranin A; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; CGA; Betagranin (N-Terminal Fragment Of Chromogranin A); Chromogranin-A
- Gene ID
- 1113
- UniProt ID
- P10645
- Sequence Similarities
- Belongs to the chromogranin/secretogranin protein family.
- Cellular Localization
- Cytoplasmic vesicle, Secreted
- Post Translation Modifications
- Sulfated on tyrosine residues and/or contains sulfated glycans., O-glycosylated with core 1 or possibly core 8 glycans., Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584)., Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).
- Protein Refseq
- NP_001266.1; NP_001288619.1
- Function
- Pancreastatin:
Strongly inhibits glucose induced insulin release from the pancreas.
Catestatin:
Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist.
Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa.
Can induce mast cell migration, degranulation and production of cytokines and chemokines.
Acts as a potent scavenger of free radicals in vitro.
May play a role in the regulation of cardiac function and blood pressure.
Serpinin:
Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CHGA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1315z | Mouse Anti-CHGA Recombinant Antibody (clone 22C4) | FC, ICC, IF, IHC | Mouse IgG2a, κ |
| MRO-0362-CN | Rabbit Anti-CHGA Recombinant Antibody (clone JM103-7) | WB, IF, IHC | Rabbit IgG |
| MRO-0363-CN | Mouse Anti-CHGA Recombinant Antibody (clone D11-2) | WB, IHC, IF | Mouse IgG2a |
| VS3-FY2 | Recombinant Mouse Anti-CHGA Antibody (clone 1B7A4) | ELISA, FC, IHC | Mouse IgG1 |
| VS3-FY3 | Recombinant Mouse Anti-CHGA Antibody (clone 5H10B12) | ELISA, WB, FC, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0690 | Hi-Affi™ Rabbit Anti-CHGA Recombinant Antibody (clone DS690AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY123 | Mouse Anti-NHP CHGA Recombinant Antibody (clone LK2H10) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY447 | Anti-CHGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY1434 | Anti-Mouse CHGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY1433 | Anti-Human CHGA Immunohistochemistry Kit | IHC |
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