Rabbit Anti-CHGA Recombinant Antibody (clone R04-3B5) (CAT#: VS3-XY3315)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human CHGA

• Species Reactivity
• Human

• Clone
• R04-3B5

• Applications
• WB, IHC-P, ICC, IF, FC

• Related Disease
• Acinar Cell Carcinoma; Neuroendocrine Carcinoma

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100
  IF: 1/50-1/200
  FC: 1/50-1/100

• Alternative Names
• CGA; PHE5; PHES

• Gene ID
• 1113

• UniProt ID
• P10645

• Long Name
• Chromogranin A

• Sequence Similarities
• Belongs to the chromogranin/secretogranin protein family.

• Cellular Localization
• Cytoplasmic vesicle, Secreted

• Post Translation Modifications
• Sulfated on tyrosine residues and/or contains sulfated glycans.
  O-glycosylated with core 1 or possibly core 8 glycans.
  Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584).
  Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).

• Protein Refseq
• NP_001266.1; NP_001288619.1

• Function
• Pancreastatin:
  Strongly inhibits glucose induced insulin release from the pancreas.
  Catestatin:
  Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220).
  Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458).
  Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543).
  Acts as a potent scavenger of free radicals in vitro (PubMed:24723458).
  May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
  Serpinin:
  Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody