Rabbit Anti-CHGA Recombinant Antibody (clone R04-3B5)
CAT#: VS3-XY3315
The recombinant rabbit antibody is specific for CHGA (Chromogranin A), which plays a role in the regulation of cardiac function and blood pressure. CHGA can induce mast cell migration, degranulation and production of cytokines and chemokines. The antibody specifically reacts with human CHGA. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17369/1734_B3_2_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23/250_A_8_3.jpg
Normal Tissue
Figure 3 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17369/141310_A_3_3.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100604-CHGA
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CHGA
- Species Reactivity
- Human
- Clone
- R04-3B5
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Acinar Cell Carcinoma; Neuroendocrine Carcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- CGA; PHE5; PHES
- Gene ID
- 1113
- UniProt ID
- P10645
- Long Name
- Chromogranin A
- Sequence Similarities
- Belongs to the chromogranin/secretogranin protein family.
- Cellular Localization
- Cytoplasmic vesicle, Secreted
- Post Translation Modifications
- Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584).
Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).
- Protein Refseq
- NP_001266.1; NP_001288619.1
- Function
- Pancreastatin:
Strongly inhibits glucose induced insulin release from the pancreas.
Catestatin:
Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220).
Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458).
Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543).
Acts as a potent scavenger of free radicals in vitro (PubMed:24723458).
May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
Serpinin:
Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CHGA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0298MZ | Recombinant Mouse Anti-Human Chromogranin A Antibody (clone EAK-A4) | IHC | Mouse antibody |
| ZG-1042F | Mouse Anti-CHGA Recombinant Antibody (clone LK2H10) | IHC-Fr, IHC-P | Mouse IgG1 |
| VS3-CJ571 | Mouse Anti-CHGA Recombinant Antibody (clone ABT-CGA) | IHC-P | Mouse IgG3, κ |
| VS3-CJ572 | Mouse Anti-CHGA Recombinant Antibody (clone PT1670) | IHC-P | Mouse IgG1, κ |
| VS3-FY1685 | Rabbit Anti-CHGA Recombinant Antibody (clone R01-7E1) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0690 | Hi-Affi™ Rabbit Anti-CHGA Recombinant Antibody (clone DS690AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY123 | Mouse Anti-NHP CHGA Recombinant Antibody (clone LK2H10) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY447 | Anti-CHGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY1434 | Anti-Mouse CHGA Immunohistochemistry Kit | IHC | |
| VS-0525-XY1433 | Anti-Human CHGA Immunohistochemistry Kit | IHC |
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