Rabbit Anti-CHRNA4 Recombinant Antibody (clone R03-1A5)
CAT#: VS3-FY2047
This is a rabbit recombinant monoclonal antibody that detects human CHRNA4. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IP. For detection of human CHRNA4 using immunoprecipitation, please refer to clone R03-1A5.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34064/70741_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101204-CHRNA4
❮
❯
WB
Figure 1 Anti-CHRNA4 Antibody (VS3-FY2047) in WB.
The Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 expression in 3T3 lysates were detected using the anti-Nicotinic Acetylcholine Receptor alpha 4 by western blot assay.
WB
Figure 2 Anti-CHRNA4 Antibody (VS3-FY2047) in WB.
The Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 expression in Hela, CHO-K1, C6, Ramos lysates were detected using the anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 by western blot assay.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human nicotinic acetylcholine receptor α4/CHRNA4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster CHRNA4
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R03-1A5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Epilepsy, Nocturnal Frontal Lobe, 1 and Autosomal Dominant Nocturnal Frontal Lobe Epilepsy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cholinergic Receptor Nicotinic Alpha 4 Subunit; Cholinergic Receptor, Nicotinic, Alpha Polypeptide 4; Cholinergic Receptor, Nicotinic, Alpha 4 (Neuronal); Cholinergic Receptor, Nicotinic Alpha 4; NACRA4; Neuronal Nicotinic Acetylcholine Receptor Alpha-4 Subunit; Acetylcholine Receptor, Nicotinic, Alpha 4 (Neuronal)
- Gene ID
- 1137
- UniProt ID
- P43681
- Sequence Similarities
- Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
- Cellular Localization
- Cell membrane, Membrane, Postsynaptic cell membrane, Synapse
- Post Translation Modifications
- Glycosylation at Asn57, Asn174, and Asn107 (NX_P43681 [NX_P43681-1])
Modification sites at PhosphoSitePlus (P43681)
Glycosylation from GlyGen (P43681) 3 sites
- Protein Refseq
- NP_000735.1; NP_001243502.1
- Function
- After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2047. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CHRNA4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3787z | Mouse Anti-CHRNA4 Recombinant Antibody (clone 19G3) | WB, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0698 | Hi-Affi™ Rabbit Anti-CHRNA4 Recombinant Antibody (clone DS698AB) | WB, IP | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
-YJ-3.jpg)
Application: ELISA
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
